裂殖酵母核糖体DNA结合因子的特性：其组分与上游激活因子及SWI/SNF亚基具有同源性。
Characterization of the fission yeast ribosomal DNA binding factor: components share homology with Upstream Activating Factor and with SWI/SNF subunits.
作者信息
Liu Meilin, Guo Ailan, Boukhgalter Boris, Van Den Heuvel Kaitlin, Tripp Matthew, Pape Louise
机构信息
Department of Chemistry, New York University, New York, NY 10003, USA.
出版信息
Nucleic Acids Res. 2002 Dec 15;30(24):5347-59. doi: 10.1093/nar/gkf683.
A ribosomal DNA (rDNA) binding activity was previously characterized in fission yeast that recognized the upstream ribosomal RNA (rRNA) gene promoter in a sequence specific manner and which stimulated rRNA synthesis. It was found to share characteristics with Saccharomyces cerevisiae's Upstream Activating Factor (UAF), an RNA polymerase I (pol I) specific transcription stimulatory factor. Putative fission yeast homologs of the S.cerevisiae UAF subunits, Rrn5p and Rrn10p, were identified. The Schizosaccharomyces pombe rDNA binding activity/transcriptional stimulatory activity was found to co-fractionate with both SpRrn5h and SpRrn10h. Analysis of polypeptides interacting with SpRrn10h uncovered a 27 kDa polypeptide (Spp27) homologous to a SWI/SNF component (now known to be homologous to Uaf30p). The contributions of the S.pombe and S.cerevisiae upstream rDNA promoter domains were assessed in cross-species transcriptional assays. Furthermore, comparative genomic analysis revealed putative Rrn5p, Rrn10p, Rrn9p and p27 homologs in multiple non-vertebrates. The S.pombe rDNA binding activity is proposed to be an RNA pol I specific SWI/SNF type factor.
先前在裂殖酵母中鉴定出一种核糖体DNA（rDNA）结合活性，它以序列特异性方式识别上游核糖体RNA（rRNA）基因启动子，并刺激rRNA合成。发现它与酿酒酵母的上游激活因子（UAF）具有共同特征，UAF是一种RNA聚合酶I（pol I）特异性转录刺激因子。鉴定出了酿酒酵母UAF亚基Rrn5p和Rrn10p的推定裂殖酵母同源物。发现粟酒裂殖酵母rDNA结合活性/转录刺激活性与SpRrn5h和SpRrn10h共分离。对与SpRrn10h相互作用的多肽的分析揭示了一种与SWI/SNF组分（现在已知与Uaf30p同源）同源的27 kDa多肽（Spp27）。在跨物种转录分析中评估了粟酒裂殖酵母和酿酒酵母上游rDNA启动子结构域的作用。此外，比较基因组分析揭示了多种非脊椎动物中推定的Rrn5p、Rrn10p、Rrn9p和p27同源物。粟酒裂殖酵母rDNA结合活性被认为是一种RNA pol I特异性SWI/SNF型因子。
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