Lie-Injo L
J Chromatogr. 1976 Feb 4;117(1):53-8. doi: 10.1016/s0021-9673(00)81065-1.
A simple method for the isolation and purification of hemoglobin components from starch gel by electrophoresis is described. An inverted bottle with a cut off bottom is used. Pieces of gel containing the hemoglobin are embedded in potato starch, wetted with buffer, and placed in the neck of the inverted bottle, the mouth of which has been closed with filter paper and tape. Connection with the negative tank buffer is achieved by inserting a layer of starch gel between the potato starch and the negative tank buffer. By electrophoresis, the hemoglobin migrates and accumulates in a dialysing tube tied around the mouth of the bottle and hanging into the positive tank buffer.
本文描述了一种通过电泳从淀粉凝胶中分离和纯化血红蛋白成分的简单方法。使用一个底部被切掉的倒置瓶子。将含有血红蛋白的凝胶块嵌入马铃薯淀粉中，用缓冲液湿润，然后放置在倒置瓶子的颈部，瓶子的口部已用滤纸和胶带封闭。通过在马铃薯淀粉和负极槽缓冲液之间插入一层淀粉凝胶来实现与负极槽缓冲液的连接。通过电泳，血红蛋白迁移并积聚在绑在瓶口并悬垂于正极槽缓冲液中的透析管中。
