麦芽糖结合蛋白作为一种溶解度增强剂。 - Suppr

Suppr

超能文献

Maltose-binding protein as a solubility enhancer.

作者信息

Fox Jeffrey D, Waugh David S

机构信息

Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.

出版信息

Methods Mol Biol. 2003;205:99-117. doi: 10.1385/1-59259-301-1:99.

DOI:10.1385/1-59259-301-1:99

PMID:12491882

Abstract

摘要

相似文献

Maltose-binding protein as a solubility enhancer.

Methods Mol Biol. 2003;205:99-117. doi: 10.1385/1-59259-301-1:99.

Fusions to maltose-binding protein: control of folding and solubility in protein purification.

Methods Enzymol. 2000;326:312-21. doi: 10.1016/s0076-6879(00)26062-x.

Assessment of protein folding/solubility in live cells.

Methods Mol Biol. 2003;205:155-69. doi: 10.1385/1-59259-301-1:155.

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.

Gene. 1988 Jul 15;67(1):21-30. doi: 10.1016/0378-1119(88)90004-2.

MBP fusion protein with a viral protease cleavage site: one-step cleavage/purification of insoluble proteins.

Biotechniques. 2001 Jun;30(6):1194-8. doi: 10.2144/01306bm01.

High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system.

Protein Expr Purif. 1997 Aug;10(3):309-19. doi: 10.1006/prep.1997.0759.

Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli.

Methods Mol Biol. 2009;498:157-72. doi: 10.1007/978-1-59745-196-3_11.

Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin.

Protein Expr Purif. 1998 Feb;12(1):122-32. doi: 10.1006/prep.1997.0826.

Purification and activity of a wheat 9-kDa lipid transfer protein expressed in Escherichia coli as a fusion with the maltose binding protein.

Protein Expr Purif. 1995 Oct;6(5):597-603. doi: 10.1006/prep.1995.1078.

Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.

Protein Sci. 2005 Dec;14(12):2964-71. doi: 10.1110/ps.051718605.

引用本文的文献

Challenges and Solutions for Leave-One-Out Biosensor Design in the Context of a Rugged Fitness Landscape.

Sensors (Basel). 2024 Oct 1;24(19):6380. doi: 10.3390/s24196380.

Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.

Nat Commun. 2024 Aug 7;15(1):6717. doi: 10.1038/s41467-024-50858-0.

Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.

bioRxiv. 2024 Jan 17:2024.01.16.575860. doi: 10.1101/2024.01.16.575860.

TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury.

Nat Neurosci. 2022 Dec;25(12):1608-1625. doi: 10.1038/s41593-022-01199-y. Epub 2022 Nov 24.

Purity of maltose-binding protein - Recombinant streptavidin expressed in BL21 (pD861-MBP: 327892).

J Adv Pharm Technol Res. 2022 Apr-Jun;13(2):117-122. doi: 10.4103/japtr.japtr_371_21. Epub 2022 Apr 7.

Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies.

Microb Cell Fact. 2022 Apr 2;21(1):51. doi: 10.1186/s12934-022-01776-7.

Use of tandem affinity-buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins.

Methods Enzymol. 2021;659:37-70. doi: 10.1016/bs.mie.2021.07.007. Epub 2021 Sep 23.

Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry.

Biochemistry. 2021 Jun 22;60(24):1876-1884. doi: 10.1021/acs.biochem.1c00138. Epub 2021 Jun 8.

Specificity Studies of the Venezuelan Equine Encephalitis Virus Non-Structural Protein 2 Protease Using Recombinant Fluorescent Substrates.

Int J Mol Sci. 2020 Oct 16;21(20):7686. doi: 10.3390/ijms21207686.

Structural basis for the evolution of cyclic phosphodiesterase activity in the U6 snRNA exoribonuclease Usb1.

Nucleic Acids Res. 2020 Feb 20;48(3):1423-1434. doi: 10.1093/nar/gkz1177.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

Maltose-binding protein as a solubility enhancer.

作者信息

Fox Jeffrey D, Waugh David S

机构信息

Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.

出版信息

Methods Mol Biol. 2003;205:99-117. doi: 10.1385/1-59259-301-1:99.

DOI:10.1385/1-59259-301-1:99

PMID:12491882

Abstract

摘要

相似文献

Maltose-binding protein as a solubility enhancer.

Methods Mol Biol. 2003;205:99-117. doi: 10.1385/1-59259-301-1:99.

Fusions to maltose-binding protein: control of folding and solubility in protein purification.

Methods Enzymol. 2000;326:312-21. doi: 10.1016/s0076-6879(00)26062-x.

Assessment of protein folding/solubility in live cells.

Methods Mol Biol. 2003;205:155-69. doi: 10.1385/1-59259-301-1:155.

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.

Gene. 1988 Jul 15;67(1):21-30. doi: 10.1016/0378-1119(88)90004-2.

MBP fusion protein with a viral protease cleavage site: one-step cleavage/purification of insoluble proteins.

Biotechniques. 2001 Jun;30(6):1194-8. doi: 10.2144/01306bm01.

High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system.

Protein Expr Purif. 1997 Aug;10(3):309-19. doi: 10.1006/prep.1997.0759.

Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli.

Methods Mol Biol. 2009;498:157-72. doi: 10.1007/978-1-59745-196-3_11.

Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin.

Protein Expr Purif. 1998 Feb;12(1):122-32. doi: 10.1006/prep.1997.0826.

Purification and activity of a wheat 9-kDa lipid transfer protein expressed in Escherichia coli as a fusion with the maltose binding protein.

Protein Expr Purif. 1995 Oct;6(5):597-603. doi: 10.1006/prep.1995.1078.

Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.

Protein Sci. 2005 Dec;14(12):2964-71. doi: 10.1110/ps.051718605.

引用本文的文献

Challenges and Solutions for Leave-One-Out Biosensor Design in the Context of a Rugged Fitness Landscape.

Sensors (Basel). 2024 Oct 1;24(19):6380. doi: 10.3390/s24196380.

Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.

Nat Commun. 2024 Aug 7;15(1):6717. doi: 10.1038/s41467-024-50858-0.

Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.

bioRxiv. 2024 Jan 17:2024.01.16.575860. doi: 10.1101/2024.01.16.575860.

TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury.

Nat Neurosci. 2022 Dec;25(12):1608-1625. doi: 10.1038/s41593-022-01199-y. Epub 2022 Nov 24.

Purity of maltose-binding protein - Recombinant streptavidin expressed in BL21 (pD861-MBP: 327892).

J Adv Pharm Technol Res. 2022 Apr-Jun;13(2):117-122. doi: 10.4103/japtr.japtr_371_21. Epub 2022 Apr 7.

Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies.

Microb Cell Fact. 2022 Apr 2;21(1):51. doi: 10.1186/s12934-022-01776-7.

Use of tandem affinity-buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins.

Methods Enzymol. 2021;659:37-70. doi: 10.1016/bs.mie.2021.07.007. Epub 2021 Sep 23.

Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry.

Biochemistry. 2021 Jun 22;60(24):1876-1884. doi: 10.1021/acs.biochem.1c00138. Epub 2021 Jun 8.

Specificity Studies of the Venezuelan Equine Encephalitis Virus Non-Structural Protein 2 Protease Using Recombinant Fluorescent Substrates.

Int J Mol Sci. 2020 Oct 16;21(20):7686. doi: 10.3390/ijms21207686.

Structural basis for the evolution of cyclic phosphodiesterase activity in the U6 snRNA exoribonuclease Usb1.

Nucleic Acids Res. 2020 Feb 20;48(3):1423-1434. doi: 10.1093/nar/gkz1177.