Joo P K, Heitmann R N, Reulein S S, Bergman E N
J Dairy Sci. 1976 Feb;59(2):309-14. doi: 10.3168/jds.S0022-0302(76)84200-2.
A method is presented for single-column analysis of the concentrations and specific activities of the free amino acids in both whole blood and plasma. Interference from glutathione in whole blood was eliminated by the use of sodium sulfite although losses of about one-half of the cystine and methionine occurred. Seventy-five percent +/- 1 of the glutamine was recovered consistently in both whole blood and plasma so that corrections for this loss readily could be made. Elimination of the baseline shift due to ammonia was accomplished by passing the buffers through ion-exchange columns before entering the sample loops. There were several significant differences between amino acid concentrations and specific activities in whole blood and in plasma, indicating that care should be taken in interpreting data on metabolism of amino acids.
本文介绍了一种用于单柱分析全血和血浆中游离氨基酸浓度及比活性的方法。使用亚硫酸钠消除了全血中谷胱甘肽的干扰，不过胱氨酸和蛋氨酸损失了约一半。全血和血浆中谷氨酰胺的回收率始终为75%±1%，因此可以很容易地对这种损失进行校正。通过使缓冲液在进入样品环之前通过离子交换柱，消除了氨引起的基线漂移。全血和血浆中的氨基酸浓度及比活性存在若干显著差异，这表明在解释氨基酸代谢数据时应谨慎。
