Nielsen N O, Bloch P, Simonsen P E
Danish Bilharziasis Laboratory (DBL), Jaegersborg Allé 1D, 2920 Charlottenlund, Denmark.
Trans R Soc Trop Med Hyg. 2002 Jul-Aug;96(4):453-8. doi: 10.1016/s0035-9203(02)90392-5.
The filarial-specific humoral responses (IgG1, IgG2, IgG3, IgG4 and IgE) to a Brugia pahangi antigen was assessed in 9 groups of adult individuals from a Wuchereria bancrofti-endemic area in north-east Tanzania. In 5 of the groups, individuals were negative for microfilariae (mf) and circulating filarial antigen (CFA) and had leg lymphoedema of varying severity ranging from early to more advanced grades. A 6th group had mixed grades of lymphoedema and were actively infected with mf and/or CFA. Three groups of asymptomatic individuals with different infection status (mf+CFA+; mf-CFA+; mf-CFA-) were also included. No differences in the antibody levels were observed between the 5 uninfected pathology groups. However, groups with advanced lymphoedema had a significantly higher level of IgG3 as compared to groups with early lymphoedema. A decline in the IgG4/IgE ratios were observed when moving from groups with early to groups with more advanced lymphoedema, which could indicate that increasing levels of IgE relatively to IgG4 are associated with progression of pathology. When all study groups were compared, higher IgG4/IgE ratios were observed in infected groups than in uninfected groups. This could suggest that high levels of IgG4 relative to IgE protect the parasite, whereas the opposite may play a role in parasite killing. When relating IgG4/IgE ratios to levels of gamma interferon (IFN gamma), a clear inverse relationship was observed. Thus, high levels of IFN gamma were found in groups with low IgG4/IgE ratios (uninfected groups) and low levels of IFN gamma were found in groups with high IgG4/IgE ratios (infected groups). The relationship between cellular (IFN gamma) and humoral (IgG4/IgE ratios) responses and their possible role in parasite protection and killing, and in development of early lymphoedema, are discussed.
在坦桑尼亚东北部班氏吴策线虫流行地区的9组成年个体中，评估了对彭亨布鲁线虫抗原的丝虫特异性体液免疫反应（IgG1、IgG2、IgG3、IgG4和IgE）。在其中5组中，个体无微丝蚴（mf）和循环丝虫抗原（CFA），患有不同严重程度的腿部淋巴水肿，从早期到更严重阶段不等。第6组有混合程度的淋巴水肿，并且被mf和/或CFA主动感染。还纳入了3组具有不同感染状态（mf+CFA+；mf-CFA+；mf-CFA-）的无症状个体。在5个未感染的病理组之间未观察到抗体水平的差异。然而，与早期淋巴水肿组相比，晚期淋巴水肿组的IgG3水平显著更高。当从早期淋巴水肿组过渡到更晚期淋巴水肿组时，观察到IgG4/IgE比值下降，这可能表明相对于IgG4，IgE水平升高与病理进展相关。当比较所有研究组时，感染组的IgG4/IgE比值高于未感染组。这可能表明相对于IgE，高水平的IgG4保护寄生虫，而相反情况可能在杀死寄生虫中起作用。当将IgG4/IgE比值与γ干扰素（IFNγ）水平相关联时，观察到明显的负相关关系。因此，在IgG4/IgE比值低的组（未感染组）中发现高水平的IFNγ，而在IgG4/IgE比值高的组（感染组）中发现低水平的IFNγ。讨论了细胞（IFNγ）和体液（IgG4/IgE比值）反应之间的关系及其在寄生虫保护和杀伤以及早期淋巴水肿发展中的可能作用。
