Kliber J S, Hoa G H, Douzou P, Graffe M, Grunberg-Manago M
Nucleic Acids Res. 1976 Dec;3(12):3423-38. doi: 10.1093/nar/3.12.3423.
Potentiometric studies of ribosomal particles 30S, 50S, and 70S, were designed to investigate possible implications of the electrostatic potentials developed by the 16S and 23S rRNA fractions. Release of protons and proton titrations of these ribosomal fractions were examined as a function of Mg2+ and K+ concentrations. The effects of these cations fit the polyelectrolyte theory remarkably well and are discussed accordingly.
对30S、50S和70S核糖体颗粒进行电位测定研究,旨在探究16S和23S rRNA组分所产生的静电势可能具有的影响。研究了这些核糖体组分的质子释放和质子滴定与Mg2+和K+浓度的关系。这些阳离子的作用与聚电解质理论非常吻合,并据此进行了讨论。