大肠杆菌核糖体及亚基中核糖体RNA 3'末端可及性的荧光研究。
Fluorescence studies of the accessibility of the 3' ends of the ribosomal RNAs in Escherichia coli ribosomes and subunits.
作者信息
Schreiber J P, Hsiung N, Cantor C R
出版信息
Nucleic Acids Res. 1979 Jan;6(1):181-93. doi: 10.1093/nar/6.1.181.
Abstract
The accessibility of the 3'-ends of E. coli in various states has been probed by reaction, after periodate oxidation, with the fluorescent dye proflavine semicarbazide. Free oxidized 16S and 23S rRNAs each react with 2 equivalents of dye. The 23S rRNA is equally reactive in the 50S subunit and the 70S ribosome. The 16S RRNA 3'-end is accessible in the 30S subunit. In the intact 70S particle, periodate can reach the 3'-end of the 16S rRNA but the dye cannot. The 5S rRNA is relatively inaccessible to periodate oxidation or dye reaction in the 70S particle. Dye-labelled 16S rRNA will reconstitute into 30S particles but they are inactive in polypeptide synthesis. This is apparently due to the inability of the 30S particles to form tight complexes with 50S subunits. Iodide quenching studies indicate that the environment of the 3'-end of 16S rRNA in the 30S particle is different from that of the free rRNA.
摘要
通过在高碘酸盐氧化后与荧光染料硫酸普罗黄素反应，对处于各种状态的大肠杆菌3'端的可及性进行了探测。游离的氧化16S和23S rRNA各自与2当量的染料反应。23S rRNA在50S亚基和70S核糖体中的反应性相同。16S rRNA的3'端在30S亚基中是可及的。在完整的70S颗粒中，高碘酸盐可以到达16S rRNA的3'端，但染料不能。在70S颗粒中，5S rRNA相对不易被高碘酸盐氧化或与染料反应。用染料标记的16S rRNA将重新组装成30S颗粒，但它们在多肽合成中无活性。这显然是由于30S颗粒无法与50S亚基形成紧密复合物。碘淬灭研究表明，30S颗粒中16S rRNA 3'端的环境与游离rRNA的不同。
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