Bodó S, Laczkó L, Horváth Gabriella, Baranyai B, Szabó Mária Horvai, Dohy J, Gócza Elen
Agricultural Biotechnology Centre, H-2100 Gödölló, P.O. Box 411, Hungary.
Acta Vet Hung. 2002;50(4):469-79. doi: 10.1556/AVet.50.2002.4.9.
This article presents a new, simple and rapid embryo biopsy method. The blastomere for genetic analysis can be separated from a precompacted mouse embryo after a partial zona digestion with the use of a holding pipette. For the micromanipulation only two microcapillaries and micromanipulators are needed. The development of the biopsied embryos was studied during in vitro culture and in utero following embryo transfer. There was no significant difference between the treated and the control groups in the ratio of embryos that developed to the blastocyst stage, although the biopsied embryos were delayed in their development because they contained significantly fewer cells compared to the control ones at the same stage. Although there was no difference in the ratio of implantation, the development of the biopsied embryos in utero was also delayed 12-24 hours on the 9th day of pregnancy. No difference in development was visible from the 13th day of pregnancy. Statistically, no differences were found in the developmental ratio (number of developed fetuses/transferred embryos) of the control and treated embryos during gastrulation (9th day of pregnancy), at the beginning of organogenesis (13th day of pregnancy) and before birth (19th day of pregnancy). The embryo biopsy method presented here can be a new and useful tool for preimplantation genetic diagnosis.
本文介绍了一种新的、简单且快速的胚胎活检方法。在使用持卵吸管对透明带进行部分消化后，可从紧实前的小鼠胚胎中分离出用于遗传分析的卵裂球。显微操作仅需两根微毛细管和显微操作器。在体外培养和胚胎移植后的子宫内对活检胚胎的发育情况进行了研究。尽管活检胚胎发育延迟，因为与同一阶段的对照胚胎相比，其细胞数量明显较少，但在发育到囊胚阶段的胚胎比例方面，处理组与对照组之间没有显著差异。尽管着床率没有差异，但在妊娠第9天，活检胚胎在子宫内的发育也延迟了12 - 24小时。从妊娠第13天开始，发育情况没有差异。统计学上，在原肠胚形成期（妊娠第9天）、器官发生开始时（妊娠第13天）和出生前（妊娠第19天），对照胚胎和处理胚胎的发育率（发育胎儿数/移植胚胎数）没有差异。本文介绍的胚胎活检方法可能成为植入前基因诊断的一种新的有用工具。
