Okada K, Laudenbach I, Schroeder F H
J Urol. 1976 Feb;115(2):164-7. doi: 10.1016/s0022-5347(17)59116-8.
The permanent epithelial strain EB 33 was initiated from primary cultures of a human prostatic adenocarcinoma in June 1973. To establish androgen dependence of growth EB 33 cells were grown in various media. The effect of androgen withdrawal was studied by the application of media containing sera that had undergone steroid extraction procedures with dichloromethane and activated charcoal. The growth rate of the original EB 33 population was not influenced by withdrawal or addition of androgenic hormones. There were 111 clones developed by single cell plating of EB 33 cells and 23 clonal lines responded to extracted media with a depression of growth of at least 50 per cent. These findings were verified in growth curve experiments. Testosterone and 5-alpha-dihydrotestosterone slightly accelerated growth but did not fully compensate the depression of cell counts seen in extracted media. These findings may be consistent with hormone dependence of growth of clonal lines developed from the human prostatic epithelial strain EB 33.
永久性上皮细胞系EB 33于1973年6月从人前列腺腺癌的原代培养物中建立。为确定EB 33细胞生长的雄激素依赖性，将其在多种培养基中培养。通过应用含有经二氯甲烷和活性炭进行类固醇提取程序的血清的培养基，研究了雄激素撤除的影响。原始EB 33细胞群体的生长速率不受雄激素激素撤除或添加的影响。通过EB 33细胞的单细胞平板接种培养出111个克隆，其中23个克隆系对提取的培养基有反应，生长至少降低50%。这些发现通过生长曲线实验得到验证。睾酮和5-α-二氢睾酮略微加速了生长，但并未完全补偿在提取的培养基中所见的细胞计数降低。这些发现可能与人前列腺上皮细胞系EB 33衍生的克隆系生长的激素依赖性一致。
