核因子-κB抑制剂可消除绵羊离体肺动脉中的缺氧性血管收缩。

Nuclear factor-kappaB inhibitors abolish hypoxic vasoconstriction in sheep-isolated pulmonary arteries.

作者信息

Uzun Ozge, Demiryürek A Tuncay

机构信息

Department of Pharmacology, Faculty of Medicine, Abant izzet Baysal University, 14450 Konuralp, Düzce, Turkey.

出版信息

Eur J Pharmacol. 2003 Jan 1;458(1-2):171-4. doi: 10.1016/s0014-2999(02)02761-9.

DOI:10.1016/s0014-2999(02)02761-9

PMID:12498922

Abstract

The aim of this study was to determine the role of nuclear factor-kappaB (NF-kappaB) in hypoxic constriction of isolated pulmonary arteries. Rings were suspended in an organ bath filled with Krebs-Henseleit solution and isometric contractions were recorded continuously. Hypoxia (%95 N(2)-%5 CO(2)) had no marked effect on resting force in artery rings. However, hypoxia caused further contractions in serotonin-precontracted arteries. Hypoxia-induced vasoconstrictions were abolished by preincubation with NF-kappaB inhibitors, pyrrolidine dithiocarbamate (100 microM) or pyrithione (10 microM). These results suggest that reactive oxygen species and/or NF-kappaB activation may be involved in the hypoxia-induced vasoconstriction in sheep-isolated pulmonary arteries.

摘要

本研究的目的是确定核因子-κB（NF-κB）在离体肺动脉缺氧性收缩中的作用。将血管环悬挂于充满 Krebs-Henseleit 溶液的器官浴槽中，并连续记录等长收缩。缺氧（95% N₂ - 5% CO₂）对动脉环的静息张力无明显影响。然而，缺氧会使血清素预收缩的动脉进一步收缩。用 NF-κB 抑制剂吡咯烷二硫代氨基甲酸盐（100 μM）或巯氧吡啶（10 μM）预孵育可消除缺氧诱导的血管收缩。这些结果表明，活性氧和/或 NF-κB 激活可能参与绵羊离体肺动脉的缺氧诱导血管收缩。