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靶向结核分枝杆菌细胞壁合成的药物：基于微孔板的UDP-吡喃半乳糖变位酶筛选方法的开发及从尿苷类文库中鉴定出一种抑制剂

Drug targeting Mycobacterium tuberculosis cell wall synthesis: development of a microtiter plate-based screen for UDP-galactopyranose mutase and identification of an inhibitor from a uridine-based library.

作者信息

Scherman Michael S, Winans Katharine A, Stern Richard J, Jones Victoria, Bertozzi Carolyn R, McNeil Michael R

机构信息

Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.

出版信息

Antimicrob Agents Chemother. 2003 Jan;47(1):378-82. doi: 10.1128/AAC.47.1.378-382.2003.

DOI:10.1128/AAC.47.1.378-382.2003
PMID:12499218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC148999/
Abstract

A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

摘要

开发了一种用于检测结核分枝杆菌细胞壁生物合成必需酶——UDP-吡喃半乳糖变位酶的微量滴定板检测方法。该检测方法基于高碘酸盐从UDP-呋喃半乳糖而非UDP-吡喃半乳糖中释放出氚标记甲醛的原理，并用于从化学文库中鉴定一种基于尿苷的酶抑制剂。

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