Bernasconi P, Cipelletti B, Passerini L, Granata T, Antozzi C, Mantegazza R, Spreafico R
Immunology and Muscular Pathology Unit, Istituto Nazionale Neurologico Carlo Besta, Milan, Italy.
Neurology. 2002 Dec 24;59(12):1998-2001. doi: 10.1212/01.wnl.0000036912.25881.7b.
Immunoreactivity of sera from patients with Rasmussen encephalitis (RE) and patients with partial epilepsy (PE) was analyzed by immunohistoblot on rat brain sections and the staining pattern compared with that obtained with antibodies to a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid and NMDA receptors. Staining for anti-glutamate receptor 3 (GluR3) was found in 82% of patients with RE and 64% of patients with PE. Histoblot analysis showed a positive staining in GluR3- and NMDA-specific regions of rat brain, providing a comprehensive CNS immunolocalization.
通过对大鼠脑切片进行免疫印迹分析，比较了拉斯穆森脑炎（RE）患者和部分性癫痫（PE）患者血清的免疫反应性，并将染色模式与用α-氨基-3-羟基-5-甲基-4-异恶唑丙酸和NMDA受体抗体获得的染色模式进行了比较。在82%的RE患者和64%的PE患者中发现了抗谷氨酸受体3（GluR3）染色。组织印迹分析显示大鼠脑的GluR3和NMDA特异性区域呈阳性染色，提供了全面的中枢神经系统免疫定位。
