Wouters Johan, Tricot Catherine, Durbecq Virginie, Roovers Martine, Stalon Victor, Droogmans Louis
Institut de Recherches Microbiologiques JM Wiame, Belgium.
Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):152-4. doi: 10.1107/s0907444902018292. Epub 2002 Dec 19.
Thermotoga maritima TruB, an enzyme responsible for the formation of pseudouridine in tRNA, has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li(2)SO(4), 20% glycerol, 13% PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 47.39, b = 83.88, c = 98.72 A, and diffract to 2.0 A resolution using synchrotron radiation. A solution was obtained by molecular replacement using part of the recently published crystal structure of Escherichia coli TruB bound to a synthetic RNA.
嗜热栖热菌TruB是一种负责在tRNA中形成假尿苷的酶，已通过悬滴气相扩散法在100 mM柠檬酸盐pH 3.5、200 mM硫酸锂、20%甘油、13%聚乙二醇8000中进行了纯化和结晶。晶体呈现正交对称，晶胞参数a = 47.39、b = 83.88、c = 98.72 Å，并使用同步辐射衍射至2.0 Å分辨率。通过分子置换法，利用最近发表的与合成RNA结合的大肠杆菌TruB晶体结构的一部分获得了一个溶液。
