Casassa E F
Macromolecules. 1976 Jan-Feb;9(1):182-5. doi: 10.1021/ma60049a029.
Some criticisms of our theoretical treatment of the partial exclusion of flexible-chain polymers in solution from cavities of macromolecular size and its application to gel permeation chromatography are examined. In other discussion, it is confirmed by simple reasoning that the identification, explicit or implicit in various studies, of the mean projection of a polymer molecule onto a line as a characteristic dimension governing the extent of permeation of simple pores does not depend on specific molecular models. Our previous calculation of permeation by certain random-flight branched-chain species is shown to lead, incidentally, to the mean projection for these structures. From relations between the mean projection and the hydrodynamic volume of a molecule, it appears that the product of intrinsic viscosity and molecular weight is not a common calibration factor for elution of all molecular species from a gel chromatographic column, but theory and experience do support the validity of this correlation among solutes with similar molecular architecture.
我们对溶液中柔性链聚合物从大分子尺寸的空腔中部分排除的理论处理及其在凝胶渗透色谱中的应用的一些批评意见进行了审视。在其他讨论中，通过简单推理证实，在各种研究中明确或隐含地将聚合物分子在一条线上的平均投影作为控制简单孔隙渗透程度的特征尺寸，并不依赖于特定的分子模型。顺便指出，我们之前对某些无规行走支链物种渗透的计算得出了这些结构的平均投影。从平均投影与分子流体力学体积之间的关系来看，特性粘度与分子量的乘积并非从凝胶色谱柱中洗脱所有分子物种的通用校准因子，但理论和经验确实支持具有相似分子结构的溶质之间这种相关性的有效性。
