Ladygin V G
Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Biofizika. 2002 Nov-Dec;47(6):1021-31.
Chlorophyll-protein complexes of photosystems (PS), as well as ultrastructural arrangement of chloroplasts in pea leaves of the primary cultivar Torsdag and mutants chlorotica 2004 and 2014 were studied. It was shown that both mutants accumulated 80 and 55% of chlorophyll, respectively, and were able to synthesize all of four types of photosystems complexes. The value of the light-harvesting antenna in mutant 2014 was close to the control one, and in mutant 2004 it increased significantly (by 30%). These changes were caused by a proportional decrease, 40-50%, of any complexes in mutant 2014, whereas the number of PS I reaction centers in mutant 2004 decreased by 50% and the reaction centers of PS II complexes were almost completely retained. It was established that the proportional decrease of PS I and PS II complexes in mutant chlorotica 2014 was followed by a partial reduction of the entire membrane system in chloroplasts, but with a good development of both granal and intergranal sites of thylakoids. On the contrary, the loss of complexes of PS I reaction centers in mutant chlorotica 2004 led to a reduction of unstacked sites of thylakoids in chloroplasts. It was concluded that the disturbance of the lateral orientation of the membrane system of chloroplasts is associated with the loss of complexes of reaction centers of PS I, which is predominantly localized in unstacked sites of thylakoids.
对主栽品种周四豌豆叶片以及突变体chlorotica 2004和chlorotica 2014中光系统（PS）的叶绿素 - 蛋白质复合物以及叶绿体的超微结构排列进行了研究。结果表明，两个突变体分别积累了80%和55%的叶绿素，并且能够合成所有四种类型的光系统复合物。突变体2014中光捕获天线的值与对照接近，而突变体2004中该值显著增加（增加了30%）。这些变化是由突变体2014中任何复合物成比例减少40 - 50%引起的，而突变体2004中PS I反应中心的数量减少了50%，PS II复合物的反应中心几乎完全保留。已确定，突变体chlorotica 2014中PS I和PS II复合物成比例减少后，叶绿体中整个膜系统部分减少，但类囊体的基粒和基质部位发育良好。相反，突变体chlorotica 2004中PS I反应中心复合物的丧失导致叶绿体中类囊体非堆叠部位减少。得出的结论是，叶绿体膜系统横向取向的紊乱与PS I反应中心复合物的丧失有关，PS I主要位于类囊体的非堆叠部位。
