Srivastava Ajay, Heise Christina, Garg Ankush, Bell John B
Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada.
Genome. 2002 Dec;45(6):1077-82. doi: 10.1139/g02-078.
Additional vein (Adv) is a dominant mutation that affects the first wing vein in Drosophila. It also manifests a recessive lethal phenotype and is associated with a large inversion. Using a combination of genetic and cytogenetic techniques, we show that Adv interacts with engrailed (en), likely because one of the inversion breakpoints interferes with en function. Genetic interaction studies reveal that Adv is lethal in trans with various lethal alleles of en and gives an engrailed-like wing phenotype with weak alleles of en. In situ hybridization to polytene chromosomes using en cDNA demonstrates that one of the inversion breakpoints lies within the en coding region. Although the cause of the wing phenotype is not determined herein, it likely is caused by the other inversion breakpoint interfering with a different function. The characterization of this mutation could expedite studies to understand what molecular events result in the Adv phenotype and thereby provide insight into the development of the first wing vein in Drosophila.
额外翅脉（Adv）是一种显性突变，影响果蝇的第一翅脉。它还表现出隐性致死表型，并与一个大的倒位相关。通过结合遗传和细胞遗传学技术，我们表明Adv与 engrailed（en）相互作用，可能是因为其中一个倒位断点干扰了en的功能。遗传相互作用研究表明，Adv与en的各种致死等位基因反式组合时是致死的，并且与en的弱等位基因一起会产生类似 engrailed 的翅表型。使用en cDNA对多线染色体进行原位杂交表明，其中一个倒位断点位于en编码区内。尽管本文未确定翅表型的原因，但可能是由另一个倒位断点干扰了不同的功能所致。对这种突变的表征可以加快研究，以了解哪些分子事件导致了Adv表型，从而深入了解果蝇第一翅脉的发育。
