生物样品中单宁酸及其代谢物的分析方法：在大鼠单宁酸代谢中的应用

Method for analysis of tannic acid and its metabolites in biological samples: application to tannic acid metabolism in the rat.

作者信息

Nakamura Yumiko, Tsuji Sumiko, Tonogai Yasuhide

机构信息

National Institute of Health Sciences, Osaka Branch, 1-1-43 Hoenzaka, Chuo-ku, Japan.

出版信息

J Agric Food Chem. 2003 Jan 1;51(1):331-9. doi: 10.1021/jf020847+.

DOI:10.1021/jf020847+

PMID:12502429

Abstract

A new analytical method for measuring tannic acid (TA) using tannase was developed and applied to the investigation of TA metabolism in the rat following oral administration at a dose of 1.0 g/kg. The proposed method for TA determination was based on the enzymatic hydrolysis of TA to gallic acid (GA) and subsequent determination by HPLC. TA metabolites were determined by HPLC. 4-O-Methylgallic acid (4-OMGA), pyrogallol (PY), and resorcinol (RE) were detected in serum. TA was excreted into urine as GA (0.01%), 4-OMGA (0.10%), PY (0.24%), and RE (2.06%) and into feces as TA (62.74%), GA (0.19%), PY (0.02%), and RE (0.76%) within 54 h after oral administration. It was suggested that >60% of TA remained unchanged but that some was hydrolyzed to GA by tannase in the intestine and further metabolized to 4-OMGA, PY, and RE.

摘要

开发了一种使用单宁酶测定单宁酸（TA）的新分析方法，并将其应用于大鼠口服剂量为1.0 g/kg后TA代谢的研究。所提出的TA测定方法基于TA酶解为没食子酸（GA），随后通过高效液相色谱法（HPLC）进行测定。通过HPLC测定TA代谢物。在血清中检测到4-邻甲基没食子酸（4-OMGA）、连苯三酚（PY）和间苯二酚（RE）。口服给药后54小时内，TA以GA（0.01%）、4-OMGA（0.10%）、PY（0.24%）和RE（2.06%）的形式排泄到尿液中，以TA（62.74%）、GA（0.19%）、PY（0.02%）和RE（0.76%）的形式排泄到粪便中。结果表明，超过60%的TA保持不变，但有些在肠道中被单宁酶水解为GA，并进一步代谢为4-OMGA、PY和RE。