Tuna Büşra, Arısoy Pırıl, Oktay Başeğmez Hatice İmge, Baydemir Peşint Gözde
Department of Bioengineering, Adana Alparslan Türkeş Science and Technology University, Sarıçam, 01250, Adana, Türkiye.
World J Microbiol Biotechnol. 2025 Feb 4;41(2):59. doi: 10.1007/s11274-025-04266-1.
The increasing resistance of microorganisms to conventional antibiotics calls for alternative antimicrobial strategies. This study introduces a novel approach to acute wound healing by incorporating epitope-imprinted spongy cover materials with antimicrobial properties, using Tannic acid (TA) as the active agent within biocompatible cryogels imprinted with gallic acid. The spongy materials were synthesized and characterized through Fourier Transform Infrared Spectroscopy (FTIR), swelling tests, and Scanning Electron Microscopy (SEM) to assess their structural and physicochemical properties. The antimicrobial efficacy of the cryogels, loaded with 1.5, 3, 5 mg/mL of TA concentrations, was tested against Staphylococcus aureus and Escherichia coli, common pathogens in wound infections. The highest inhibition zone was determined to be 15 mm for S. aureus and 12 mm for E. coli. Maximum TA adsorption was 210.27 mg/g for eMIP and 24.74 mg/g for NIP. Cumulative release studies revealed the highest release rate occurred within the first 2 h. TA release kinetics indicated a non-Fickian diffusion mechanism. Additionally, the biocompatibility and potential cytotoxicity of the spongy materials, including TA-loaded variants, were assessed using the MTT assay on cultured cells. The results confirmed that the spongy materials are non-toxic and do not inhibit cell proliferation, supporting their suitability for acute wound healing. This study demonstrates that TA-loaded epitope-imprinted Poly(2-hydroxyethyl methacrylate) (pHEMA)-based spongy materials possess antimicrobial properties, making them potential candidates for wound and burn dressing applications.
微生物对传统抗生素的耐药性不断增加，这就需要替代的抗菌策略。本研究引入了一种急性伤口愈合的新方法，通过将具有抗菌特性的表位印迹海绵覆盖材料与作为活性剂的单宁酸（TA）相结合，TA被包载于用没食子酸印迹的生物相容性冷冻凝胶中。通过傅里叶变换红外光谱（FTIR）、溶胀试验和扫描电子显微镜（SEM）对海绵材料进行合成和表征，以评估其结构和物理化学性质。测试了负载浓度为1.5、3、5mg/mL TA的冷冻凝胶对金黄色葡萄球菌和大肠杆菌这两种伤口感染常见病原体的抗菌效果。对金黄色葡萄球菌的最大抑菌圈为15mm，对大肠杆菌为12mm。eMIP的最大TA吸附量为210.27mg/g，NIP为24.74mg/g。累积释放研究表明，最高释放速率发生在最初2小时内。TA释放动力学表明其为非菲克扩散机制。此外，使用MTT法对培养细胞评估了包括负载TA变体在内的海绵材料的生物相容性和潜在细胞毒性。结果证实，海绵材料无毒且不抑制细胞增殖，支持其适用于急性伤口愈合。本研究表明，负载TA的表位印迹聚甲基丙烯酸2-羟乙酯（pHEMA）基海绵材料具有抗菌性能，使其成为伤口和烧伤敷料应用的潜在候选材料。
