1型人类免疫缺陷病毒cDNA体外整合的辅助因子
Cofactors for human immunodeficiency virus type 1 cDNA integration in vitro.
作者信息
Gao Kui, Gorelick Robert J, Johnson Donald G, Bushman Frederic
机构信息
Infectious Disease Laboratory, The Salk Institute, La Jolla, California 92037, USA.
出版信息
J Virol. 2003 Jan;77(2):1598-603. doi: 10.1128/jvi.77.2.1598-1603.2003.
Abstract
We have investigated the function of two DNA binding proteins that stimulate human immunodeficiency virus type 1 cDNA integration in vitro, the cellular HMGa1 protein and the viral nucleocapsid (NC) protein. Of the three forms of NC (NCp7, NCp9, and NCp15), we find that NCp9 is the most effective at increasing integration in vitro; thus, processing of NC may potentially modulate its activities during infection. We also found that maximal stimulation by NCp9 required roughly enough NC to coat the reactant DNAs whereas less HMGa1 was required, and the reactions displayed different optima for divalent metal cofactors and order of addition. These findings reveal probable distinct mechanisms of action in vitro.
摘要
我们研究了两种在体外刺激人类免疫缺陷病毒1型（HIV-1）cDNA整合的DNA结合蛋白的功能，即细胞HMGa1蛋白和病毒核衣壳（NC）蛋白。在NC的三种形式（NCp7、NCp9和NCp15）中，我们发现NCp9在体外增加整合方面最有效；因此，NC的加工可能在感染过程中调节其活性。我们还发现，NCp9的最大刺激需要大致足够的NC来包裹反应性DNA，而所需的HMGa1较少，并且反应对二价金属辅因子显示出不同的最佳条件和添加顺序。这些发现揭示了体外可能不同的作用机制。
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