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1型人类免疫缺陷病毒体外整合信号序列中碱基的不同作用

Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro.

作者信息

Yoshinaga T, Fujiwara T

机构信息

Shionogi Institute for Medical Science, Osaka, Japan.

出版信息

J Virol. 1995 May;69(5):3233-6. doi: 10.1128/JVI.69.5.3233-3236.1995.

DOI:10.1128/JVI.69.5.3233-3236.1995
PMID:7707558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC189032/
Abstract

To investigate the roles of bases near the tips of each strand of the long terminal repeat of the human immunodeficiency virus type 1 in the integration reaction, we examined the efficiencies of both binding and integration activities of staggered-ended substrates and mismatched mutant substrates by the integration assay and the UV cross-linking assay. Our results suggest that some bases of the human immunodeficiency virus type 1 long terminal repeat are required primarily for binding, whereas others are more critical for later reaction steps in vitro.

摘要

为了研究人类免疫缺陷病毒1型长末端重复序列每条链末端附近的碱基在整合反应中的作用，我们通过整合试验和紫外线交联试验检测了交错末端底物和错配突变底物的结合及整合活性效率。我们的结果表明，人类免疫缺陷病毒1型长末端重复序列的一些碱基主要是结合所必需的，而其他碱基对体外后期反应步骤更为关键。

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本文引用的文献

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Human immunodeficiency virus type 1 DNA integration: fine structure target analysis using synthetic oligonucleotides.1型人类免疫缺陷病毒DNA整合：使用合成寡核苷酸的精细结构靶标分析
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Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex.鉴定HIV-1整合酶的离散功能结构域及其在活性多聚体复合物中的组织方式。
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Detection and characterization of a functional complex of human immunodeficiency virus type 1 integrase and its DNA substrate by UV cross-linking.通过紫外线交联检测和鉴定人类免疫缺陷病毒1型整合酶及其DNA底物的功能复合物
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A stable complex between integrase and viral DNA ends mediates human immunodeficiency virus integration in vitro.整合酶与病毒DNA末端之间的稳定复合物在体外介导人类免疫缺陷病毒的整合。
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