通过表面等离子体共振测定抗体与包膜病毒颗粒结合的价态。
Valency of antibody binding to enveloped virus particles as determined by surface plasmon resonance.
作者信息
Hardy Sam A, Dimmock Nigel J
机构信息
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.
出版信息
J Virol. 2003 Jan;77(2):1649-52. doi: 10.1128/jvi.77.2.1649-1652.2003.
Abstract
A simple method is described for determining the valency of binding of immunoglobulin G to immobilized influenza A virus. Where there is a free Fab arm (monovalent binding), a second virus particle is captured. This is detected by surface plasmon resonance. The methodology should be applicable to all enveloped and nonenveloped viruses.
摘要
描述了一种用于确定免疫球蛋白G与固定化甲型流感病毒结合价态的简单方法。当存在游离的Fab臂（单价结合）时，会捕获第二个病毒颗粒。这通过表面等离子体共振检测。该方法应适用于所有包膜病毒和非包膜病毒。
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