Scorpion toxins that block T-type Ca2+ channels in spermatogenic cells inhibit the sperm acrosome reaction.
作者信息
López-González Ignacio, Olamendi-Portugal Timoteo, De la Vega-Beltrán José L, Van der Walt Jurg, Dyason Karin, Possani Lourival D, Felix Ricardo, Darszon Alberto
机构信息
Department of Development Genetics and Molecular Physiology, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad 2001, Col. Chamilpa, CP 62100, Cuernavaca, Mor., Mexico.
出版信息
Biochem Biophys Res Commun. 2003 Jan 10;300(2):408-14. doi: 10.1016/s0006-291x(02)02859-0.
The acrosome reaction (AR) is a Ca(2+)-dependent event required for sperm to fertilize the egg. The activation of T-type voltage-gated Ca(2+) channels plays a key role in the induction of this process. This report describes the actions of two toxins from the scorpion Parabuthus granulatus named kurtoxin-like I and II (KLI and KLII, respectively) on sperm Ca(2+) channels. Both toxins decrease T-type Ca(2+) channel activity in mouse spermatogenic cells and inhibit the AR in mature sperm. Saturating concentrations of the toxins inhibited at most approximately 70% of the whole-cell Ca(2+) current, suggesting the presence of a toxin-resistant component. In addition, both toxins inhibited approximately 60% of the AR, which is consistent with the participation of T-type Ca(2+) channels in the sperm AR.
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