某些微量营养素和褪黑素对大鼠血浆脂质、脂质过氧化及同型半胱氨酸水平的影响。

Effects of certain micronutrients and melatonin on plasma lipid, lipid peroxidation, and homocysteine levels in rats.

作者信息

Baydas Giyasettin, Yilmaz Okkes, Celik Sait, Yasar Abdullah, Gursu M Ferit

机构信息

Department of Physiology, College of Medicine, Firat University, Elazig, Turkey.

出版信息

Arch Med Res. 2002 Nov-Dec;33(6):515-9. doi: 10.1016/s0188-4409(02)00414-9.

DOI:10.1016/s0188-4409(02)00414-9

PMID:12505094

Abstract

BACKGROUND

Numerous studies suggest an association between high intake of antioxidant vitamins and fish oil and reduced risk of coronary heart disease. Hyperhomocysteinemia has also been identified as an independent risk factor for arteriosclerosis. In this paper, we aimed to evaluate the effects of vitamin E, vitamin C, vitamin C 6 palmitate (VC6P), lipoic acid, fish oil, and melatonin supplementation on lipid peroxidation, plasma lipid, and homocysteine (Hcy) levels in rats.

METHODS

Animals were divided into seven groups: one was used as control and each remaining group was supplemented with one substance for 6 weeks. All substances were dissolved in olive oil and injected intraperitoneally (i.p.) with the exception of vitamin C, which was dissolved in drinking water. Plasma Hcy, lipid peroxidation, and lipids were determined.

RESULTS

Plasma malondialdehyde (MDA) levels decreased significantly in melatonin (p <0.01), lipoic acid (p <0.01), and vitamin E (p <0.05) groups. On the other hand, supplementation with vitamin C and VC6OP lowered MDA levels moderately but not significantly (p >0.05). Fish oil supplementation caused a slight but insignificant increase in plasma MDA levels (p >0.05). Plasma lipid levels in animals treated with melatonin, vitamin E, vitamin C, lipoic acid, and fish oil were significantly lower than those of controls; however, treatment of rats with VC6P has no significant effect on plasma lipid level. Melatonin and fish oil administration significantly lowered plasma Hcy levels, whereas VC6P elevated its level. There was no significant effect of vitamin E, vitamin C, and lipoic acid on levels of plasma Hcy.

CONCLUSIONS

Our data suggest that supplementation with antioxidants appears to be hypolipidemic. In addition to these beneficial effects, administration of melatonin and fish oil deserves careful consideration as a measure to lower plasma Hcy levels and reduce risk of cardiovascular diseases.

摘要

背景

大量研究表明，高摄入抗氧化维生素和鱼油与降低冠心病风险之间存在关联。高同型半胱氨酸血症也已被确认为动脉硬化的一个独立危险因素。在本文中，我们旨在评估补充维生素E、维生素C、维生素C 6棕榈酸酯（VC6P）、硫辛酸、鱼油和褪黑素对大鼠脂质过氧化、血脂和同型半胱氨酸（Hcy）水平的影响。

方法

将动物分为七组：一组作为对照组，其余每组补充一种物质，持续6周。除维生素C溶解于饮用水中外，所有物质均溶解于橄榄油中并腹腔注射。测定血浆Hcy、脂质过氧化和血脂水平。

结果

褪黑素组（p<0.01）、硫辛酸组（p<0.01）和维生素E组（p<0.05）的血浆丙二醛（MDA）水平显著降低。另一方面，补充维生素C和VC6OP可适度降低MDA水平，但无显著差异（p>0.05）。补充鱼油导致血浆MDA水平略有升高，但无显著差异（p>0.05）。用褪黑素、维生素E、维生素C、硫辛酸和鱼油治疗的动物的血浆脂质水平显著低于对照组；然而，用VC6P治疗大鼠对血浆脂质水平无显著影响。褪黑素和鱼油给药显著降低血浆Hcy水平，而VC6P则使其水平升高。维生素E、维生素C和硫辛酸对血浆Hcy水平无显著影响。