Nakamura Toshiyuki, Sasaki Takayuki, Fujimori Minoru, Yazawa Kazuyuki, Kano Yasunobu, Amano Jun, Taniguchi Shun'ichiro
Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
Biosci Biotechnol Biochem. 2002 Nov;66(11):2362-6. doi: 10.1271/bbb.66.2362.
Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.
长双歧杆菌是正常菌群中的一种非致病性厌氧菌。最近，有报道称长双歧杆菌在缺氧实体瘤中蓄积。通过穿梭载体pBLES100在实体瘤中转染的长双歧杆菌中表达了感兴趣的基因。在本报告中，我们构建了包含胞嘧啶脱氨酶基因的pBLES100-S-eCD。我们通过蛋白质印迹法证实转染的长双歧杆菌产生了胞嘧啶脱氨酶。此外，转染的长双歧杆菌产生的胞嘧啶脱氨酶可将5-氟胞嘧啶转化为5-氟尿嘧啶。长双歧杆菌可能对缺氧实体瘤的酶/前药治疗有用。
