与氨酰基 - tRNA 3' 末端类似的各种 2'(3') - O - 氨酰基二核苷磷酸酯的光谱性质。
Spectroscopic properties of various 2'(3')-O-aminoacyldinucleoside phosphates analogous to the 3' terminus of AA-tRNA.
作者信息
Bobst A M, Chládek S
出版信息
Nucleic Acids Res. 1976 Jan;3(1):63-8. doi: 10.1093/nar/3.1.63.
Hypochromicity and circular dichroism data are reported for the 2' and 3'-0-aminiacyldinucleoside phosphates cytidylyl-(3'-5')-2'(3')-0-L-phenylalanyl-adenosine, cytidylyl-(3'-5')-2'-deoxy-3'-0-L-phenylalanyladenosine, cytidylyl-(3'-5')-2'-deoxy-3'-0-glycyladenosine, and cytidylyl-(3'-5')-3'-deoxy-2'-0-L-phenylalanyladenosine, all of which can act as analogs of the 3' terminus of AA-tRNA in various partial reactions of protein biosynthesis. Although all these systems have a 2'-OH group in the furanose of the 3'-residue, differences exist in the extent and/or mode of base-base overlap for most of them, except for cytidylyl-(3'-5')-2'(3')-0-L-phenylalanyladenosine and cytidylyl-(3'-5')-3'-deoxy-2'-0-L-phenylalanyladenosine. It is concluded that the biological activity of the above analogs is affected both by the position of the aminoacyl group and the stacking properties of the bases.
报道了2'-和3'-O-氨酰基二核苷磷酸酯胞苷酰-(3'-5')-2'(3')-O-L-苯丙氨酰腺苷、胞苷酰-(3'-5')-2'-脱氧-3'-O-L-苯丙氨酰腺苷、胞苷酰-(3'-5')-2'-脱氧-3'-O-甘氨酰腺苷以及胞苷酰-(3'-5')-3'-脱氧-2'-O-L-苯丙氨酰腺苷的减色性和圆二色性数据，所有这些在蛋白质生物合成的各种部分反应中都可作为氨酰基-tRNA 3'末端的类似物。尽管所有这些体系在3'-残基的呋喃糖中都有一个2'-OH基团，但除了胞苷酰-(3'-5')-2'(3')-O-L-苯丙氨酰腺苷和胞苷酰-(3'-5')-3'-脱氧-2'-O-L-苯丙氨酰腺苷外，它们大多数在碱基-碱基重叠的程度和/或模式上存在差异。得出的结论是，上述类似物的生物活性受氨酰基的位置和碱基的堆积性质的影响。
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