Gizak A, Rakus D, Dzugaj A
Department of Animal Physiology, Institute of Zoology, Wroclaw Unviersity, Cybulskiego 30, 50-205 Wroclaw, Poland.
Histol Histopathol. 2003 Jan;18(1):135-42. doi: 10.14670/HH-18.135.
The localization of fructose-1,6-bisphosphatase (FBPase) in human skeletal muscle was determined immunohistochemically using polyclonal antibodies. Light microscopy analysis, confirmed with the use of confocal microscopy, indicated that the enzyme is localized on both sides of the Z line of myocytes. The immunohistochemical investigation was confirmed by a co-sedimentation experiment which revealed that muscle FBPase binds strongly to alpha-actinin--a major structural protein of the Z line. This is the first report on localization of FBPase in skeletal muscle tissue.
使用多克隆抗体通过免疫组织化学方法确定了果糖-1,6-二磷酸酶（FBPase）在人骨骼肌中的定位。通过共聚焦显微镜确认的光学显微镜分析表明，该酶定位于肌细胞Z线的两侧。通过共沉降实验证实了免疫组织化学研究，该实验表明肌肉FBPase与α-辅肌动蛋白（Z线的主要结构蛋白）强烈结合。这是关于FBPase在骨骼肌组织中定位的首次报道。
