通过连接酶检测反应和通用阵列格式检测HLA多态性：低分辨率基因分型的初步研究

Detection of HLA polymorphisms by ligase detection reaction and a universal array format: a pilot study for low resolution genotyping.

作者信息

Consolandi Clarissa, Busti Elena, Pera Cinzia, Delfino Laura, Ferrara Gian Battista, Bordoni Roberta, Castiglioni Bianca, Bernardi Luigi Rossi, Battaglia Cristina, De Bellis Gianluca

机构信息

Dipartimento di Scienze e Tecnologie Biomediche and CISI, Università degli Studi di Milano, Segrate, Italy.

出版信息

Hum Immunol. 2003 Jan;64(1):168-78. doi: 10.1016/s0198-8859(02)00685-7.

DOI:10.1016/s0198-8859(02)00685-7

PMID:12507828

Abstract

We present our results in the identification of polymorphic sites within the second exon of the human leukocyte antigen A (HLA-A) region using the DNA microarray technology. Allele specific detection was performed by polymerase chain reaction followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. By this approach we confirmed 32 human samples previously characterized by direct DNA sequencing, thus demonstrating the interest of this approach.

摘要

我们展示了使用DNA微阵列技术在人类白细胞抗原A（HLA-A）区域的第二个外显子内鉴定多态性位点的结果。通过聚合酶链反应，随后结合连接酶检测反应（LDR）与通用阵列进行等位基因特异性检测，这是一种用于高通量DNA序列分析的强大方法。通过这种方法，我们证实了之前通过直接DNA测序表征的32个人类样本，从而证明了这种方法的实用性。