Hu Shou-wang, Zhang Fan, Wang Hui, Geng Yong-yao, Wang Sheng-qi
Shenzhen Yishengtang Biological Products Co, LTD, Shenzhen 518026, China.
Zhonghua Xue Ye Xue Za Zhi. 2003 Jul;24(7):340-3.
To investigate HLA genotyping by oligonucleotide arrays.
Unsymmetrical PCR was used to amplify HLA-A gene exon 2, 3. The PCR products were used as templates for hybridization. The oligonucleotide probes were spotted on glass to make microarrays. High signal and specific probes were selected. The effects of the length and location of probes on hybridization signal were studied. A set of computer software was designed for scanning and genotype differentiation.
The genotypes of 30 samples analyzed by microarray showed concordance to that by SBT and PCR-SSP.
HLA-A genotyping by oligonucleotide array is a good method with advantage of high speed, low cost and high flux.
通过寡核苷酸阵列研究人类白细胞抗原（HLA）基因分型。
采用不对称聚合酶链反应（PCR）扩增HLA - A基因的第2、3外显子。PCR产物用作杂交模板。将寡核苷酸探针点样于玻片上制成微阵列。筛选出高信号且特异的探针。研究探针长度和位置对杂交信号的影响。设计一套计算机软件用于扫描和基因型判别。
通过微阵列分析的30个样本的基因型与序列特异性分型（SBT）和聚合酶链反应-序列特异性引物（PCR - SSP）结果一致。
寡核苷酸阵列进行HLA - A基因分型是一种良好的方法，具有速度快、成本低、通量高的优点。
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