Kochan Kelli J, Wright David A, Schroeder Lisa J, Shen Jianjun, Morizot Donald C
Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Science Park--Research Division, Smithville, Texas 78957, USA.
Dev Dyn. 2003 Jan;226(1):99-102. doi: 10.1002/dvdy.10193.
Amphibians, and particularly the African clawed frog Xenopus laevis, have been used for more than a century as models of vertebrate embryonic development. However, in many cases, elucidation of developmental functions of specific gene sequences could be severely impeded, because X. laevis is a tetraploid species, with multiple functional copies of many genes of interest. Recent studies have shifted focus to the West African or tropical clawed frog, X. tropicalis, the only known diploid species of the genus Xenopus. Here, we present two preliminary linkage maps, constructed by analysis of joint segregation of amplified fragment length polymorphism (AFLP) markers in a X. tropicalis interstrain hybrid. A total of 53 markers, including 51 AFLP markers and 2 isozyme markers, are presently assigned to 13 multipoint linkage groups on a map of the maternal strain, whereas 9 AFLP markers from the paternal strain are assigned to 3 linkage groups on a separate map. A dense genetic linkage map is essential in mapping new developmental mutants and determining their sequences by positional cloning.
两栖动物，尤其是非洲爪蟾（非洲爪蟾），在一个多世纪以来一直被用作脊椎动物胚胎发育的模型。然而，在许多情况下，特定基因序列发育功能的阐明可能会受到严重阻碍，因为非洲爪蟾是四倍体物种，许多感兴趣的基因都有多个功能拷贝。最近的研究已将重点转移到西非或热带爪蟾，即热带爪蟾，它是爪蟾属中唯一已知的二倍体物种。在这里，我们展示了两个初步的连锁图谱，这些图谱是通过分析热带爪蟾种间杂交中扩增片段长度多态性（AFLP）标记的联合分离构建而成的。目前，共有53个标记，包括51个AFLP标记和2个同工酶标记，被定位到母本菌株图谱上的13个多点连锁群中，而来自父本菌株的9个AFLP标记被定位到单独图谱上的3个连锁群中。密集的遗传连锁图谱对于定位新的发育突变体并通过定位克隆确定其序列至关重要。
