Kawazu Masahito, Kanda Yoshinobu, Goyama Susumu, Takeshita Masataka, Nannya Yasuhito, Niino Miyuki, Komeno Yukiko, Nakamoto Tetsuya, Kurokawa Mineo, Tsujino Shiho, Ogawa Seishi, Aoki Katsunori, Chiba Shigeru, Motokura Toru, Ohishi Nobuya, Hirai Hisamaru
Department of Hematology & Oncology, University of Tokyo, Tokyo, Japan.
Am J Hematol. 2003 Jan;72(1):27-30. doi: 10.1002/ajh.10259.
Polymerase chain reaction (PCR) is a sensitive method for detection of Aspergillus DNA in bronchoalveolar lavage fluid, but it has not yet been able to distinguish infection from contamination. We have established a technique to quantify Aspergillus DNA using a real-time PCR method to resolve this problem, and we report herein a successful application of real-time PCR to diagnose invasive pulmonary aspergillosis by comparing the amount of Aspergillus DNA in bronchial lavage fluid from an affected area to that from an unaffected area. This novel tool will provide rapid, sensitive, and specific diagnosis of pulmonary aspergillosis.
聚合酶链反应（PCR）是检测支气管肺泡灌洗液中曲霉菌DNA的一种灵敏方法，但它尚未能够区分感染与污染。我们建立了一种使用实时PCR方法定量曲霉菌DNA的技术来解决这一问题，并且我们在此报告通过比较患侧支气管灌洗液与未患侧支气管灌洗液中曲霉菌DNA的量，实时PCR在诊断侵袭性肺曲霉病中的成功应用。这种新工具将为肺曲霉病提供快速、灵敏且特异的诊断。
