人食管鳞状细胞癌不同阶段的基因表达谱
Gene expression profiles at different stages of human esophageal squamous cell carcinoma.
作者信息
Zhou Jin, Zhao Li-Qun, Xiong Mo-Miao, Wang Xiu-Qin, Yang Guan-Rui, Qiu Zong-Liang, Wu Min, Liu Zhi-Hua
机构信息
National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
出版信息
World J Gastroenterol. 2003 Jan;9(1):9-15. doi: 10.3748/wjg.v9.i1.9.
AIM
To characterize the gene expression profiles in different stages of carcinogenesis of esophageal epithelium.
METHODS
A microarray containing 588 cancer related genes was employed to study the gene expression profile at different stages of esophageal squamous cell carcinoma including basal cell hyperplasia, high-grade dysplasia, carcinoma in situ, early and late cancer. Principle component analysis was performed to search the genes which were important in carcinogenesis.
RESULTS
More than 100 genes were up or down regulated in esophageal epithelial cells during the stages of basal cell hyperplasia, high-grade dysplasia, carcinoma in situ, early and late cancer. Principle component analysis identified a set of genes which may play important roles in the tumor development. Comparison of expression profiles between these stages showed that some genes, such as P160ROCK, JNK2, were activated and may play an important role in early stages of carcinogenesis.
CONCLUSION
These findings provided an esophageal cancer-specific and stage-specific expression profiles, showing that complex alterations of gene expression underlie the development of malignant phenotype of esophageal cancer cells.
目的
表征食管上皮癌变不同阶段的基因表达谱。
方法
采用包含588个癌症相关基因的微阵列研究食管鳞状细胞癌不同阶段（包括基底细胞增生、高级别发育异常、原位癌、早期和晚期癌症）的基因表达谱。进行主成分分析以寻找在癌变过程中起重要作用的基因。
结果
在基底细胞增生、高级别发育异常、原位癌、早期和晚期癌症阶段，超过100个基因在食管上皮细胞中上调或下调。主成分分析确定了一组可能在肿瘤发展中起重要作用的基因。这些阶段之间的表达谱比较表明，一些基因，如P160ROCK、JNK2，被激活且可能在癌变早期起重要作用。
结论
这些发现提供了食管癌特异性和阶段特异性的表达谱，表明基因表达的复杂改变是食管癌细胞恶性表型发展的基础。
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