[吻合动脉弹性纤维的扫描电子显微镜观察]
[Scanning electronic microscope observation of elastic fiber of anastomosed artery].
作者信息
Qiu Quan-guang, Yu Hui-yang, Ke Jin-xing
机构信息
Department of Applied Anatomy and Surgery, Third Military Medical University, Chongqing, P. R. China 400038.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2002 Nov;16(6):371-3.
OBJECTIVE
To observe the architecture of elastic fiber of anastomosed artery.
METHODS
The right femoral arteries of 60 Wistar rats were cut off transversely and end-to-end anastomosis were performed. On the 3rd, 7th, 14th, 21st, 30th and 90th days after operation, the anastomosed artery segments were harvested and fixed by 10% formalin. After routine processed, the architecture of elastic fiber of anastomosed artery was observed under scanning electronic microscope and was compared with that of normal artery.
RESULTS
On the 3rd and 7th days after anastomosis, there was no the elastic fiber in the middle of the anastomosed area. From 14 to 90 days after anastomosis, the newborn elastic fiber connected the anastomosed area. The reconstruction of elastic fiber could be divided into quiescent stage, proliferation stage, and rebuilding stage.
CONCLUSION
The reconstruction of elastic fiber occurs after arterious anastomosis and newborn elastic fiber originates from endoarterious layer. The structure of elastic fiber can return to normal 30 days after anastomosis.
目的
观察吻合动脉弹性纤维的结构。
方法
将60只Wistar大鼠的右侧股动脉横断后行端端吻合。于术后第3、7、14、21、30和90天，取吻合动脉段，用10%甲醛固定。经常规处理后，在扫描电子显微镜下观察吻合动脉弹性纤维的结构，并与正常动脉进行比较。
结果
吻合后第3天和第7天，吻合区中部无弹性纤维。吻合后14至90天，新生弹性纤维连接吻合区。弹性纤维的重建可分为静止期、增殖期和重塑期。
结论
动脉吻合后发生弹性纤维重建，新生弹性纤维起源于动脉内膜层。吻合后30天弹性纤维结构可恢复正常。
Zotero 插件