Cavadas P C, Vera-Sempere F J
Department of Plastic Surgery, La Fe Universitary Hospital, Valencia, Spain.
Microsurgery. 1996;17(3):109-14. doi: 10.1002/(SICI)1098-2752(1996)17:3<109::AID-MICR1>3.0.CO;2-H.
In vivo freezing of microarteries with liquid nitrogen is known to relieve spasm without inducing thrombosis. In the present study the patency rates of microanastomoses in pre- and postfrozen vessels were investigated in the rat model. Freezing of the femoral artery was done with ethyl chloride. The artery was frozen before the anastomosis was performed on the left side (prefrozen), and after on the right side (postfrozen); patency rates were recorded at 2, 10, and 30 days. Patency rates were 100% in prefrozen vessels and 100% in postfrozen ones at all three time intervals. Histologic examination showed depopulation of all vessel walls with loss of the intima layer and fragmentation of the inner elastic lamina. Progressive cellular repopulation and regeneration of the endothelium occurred later, with no differences between pre- and postfrozen arteries. It is concluded that freezing of vessels with ethyl chloride reverts arterial spasm without inducing thrombosis and that frozen arteries can be repaired by microsurgical anastomosis with patency rates comparable to those of virgin arteries.
已知用液氮对微动脉进行体内冷冻可缓解痉挛而不诱发血栓形成。在本研究中，在大鼠模型中研究了冷冻前后血管中微吻合口的通畅率。用氯乙烷对股动脉进行冷冻。在左侧进行吻合术前（预冷冻）和右侧进行吻合术后（后冷冻）对动脉进行冷冻；在第2、10和30天记录通畅率。在所有三个时间间隔，预冷冻血管的通畅率为100%，后冷冻血管的通畅率也为100%。组织学检查显示所有血管壁细胞减少，内膜层缺失，内弹性膜断裂。随后内皮细胞逐渐重新填充和再生，预冷冻和后冷冻动脉之间无差异。结论是，用氯乙烷冷冻血管可逆转动脉痉挛而不诱发血栓形成，并且冷冻动脉可通过显微外科吻合修复，通畅率与未处理的动脉相当。
