Martin R J, Herbein J H
Proc Soc Exp Biol Med. 1976 Jan;151(1):231-5. doi: 10.3181/00379727-151-39180.
In this study of spontaneous obesity of pigs, specific metabolic shifts were observed, which explain an increase in fat deposition. Liver tissue utilization of pyruvate and glucose for oxidation and lipogenesis showed no significant difference between lean and obese pigs. Adipose tissue utilization of glucose, acetate and glycerol for triglyceride and fatty acid synthesis was greater in obese pigs than lean pigs (P less than 0.01). No significant difference in leucine incorporation into lipid fractions was found. Of the substrates utilized, glucose supplied 86 and 94% of the glyceride-glycerol synthesized in lean and obese pigs, respectively. Glycerol was not a major contributor to glyceride-glycerol synthesis (3.5 to 5.5%), in spite of the presence of adipose tissue glycerokinase. An increase (P less than 0.05) in alanine incorporation into glucose was observed in liver tissue from obese pigs. In general, the levels of enzymes activities associated with gluconeogenesis, glycolysis, and lipogenesis supported the findings of in vitro utilization of these substrates.
在这项关于猪自发性肥胖的研究中，观察到了特定的代谢变化，这些变化解释了脂肪沉积的增加。瘦猪和肥胖猪的肝脏组织利用丙酮酸和葡萄糖进行氧化及脂肪生成的情况没有显著差异。肥胖猪的脂肪组织利用葡萄糖、乙酸盐和甘油进行甘油三酯和脂肪酸合成的能力比瘦猪更强（P小于0.01）。在亮氨酸掺入脂质组分方面未发现显著差异。在所利用的底物中，葡萄糖分别为瘦猪和肥胖猪合成的甘油酯甘油提供了86%和94%。尽管存在脂肪组织甘油激酶，但甘油并非甘油酯甘油合成的主要贡献者（3.5%至5.5%）。在肥胖猪的肝脏组织中观察到丙氨酸掺入葡萄糖的量有所增加（P小于0.05）。总体而言，与糖异生、糖酵解和脂肪生成相关的酶活性水平支持了这些底物体外利用的研究结果。
