Niewiarowski S, Goldstein S
Proc Soc Exp Biol Med. 1976 Feb;151(2):253-6. doi: 10.3181/00379727-151-39185.
Thrombin stimulated human skin fibroblasts to retract fibrin clots. When Bothrops marajoensis thrombinlike enzyme was substituted for thrombin, no retraction occurred. Fibroblasts were found to contain 12 nmole of ATP and 3.6 nmole of ADP/mg of protein, a value closely resembling that of nonmetabolic adenine nucleotides in platelets. Thrombin caused neither release of adenine nucleotides from the suspension of fibroblasts harvested enzymatically nor did addition of ADP stimulate fibroblasts to retract fibrin clots.
凝血酶刺激人皮肤成纤维细胞使纤维蛋白凝块回缩。当用马拉开波矛头蝮蛇类凝血酶替代凝血酶时，未发生凝块回缩。发现成纤维细胞每毫克蛋白质含有12纳摩尔ATP和3.6纳摩尔ADP，该值与血小板中无代谢活性的腺嘌呤核苷酸的值非常相似。凝血酶既未导致酶促收获的成纤维细胞悬液中腺嘌呤核苷酸的释放，添加ADP也未刺激成纤维细胞使纤维蛋白凝块回缩。
