华支睾吸虫成虫中28 kDa谷胱甘肽S-转移酶的超微结构定位
Ultrastructural localization of 28 kDa glutathione S-transferase in adult Clonorchis sinensis.
作者信息
Hong Sung-Jong, Yu Jae-Ran, Kang Shin-Yong
机构信息
Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
出版信息
Korean J Parasitol. 2002 Dec;40(4):173-6. doi: 10.3347/kjp.2002.40.4.173.
Abstract
Glutathione S-transferase (28GST) with molecular mass of 28 kDa is an antioxidant enzyme abundant in Clonorchis sinensis. In adult C. sinensis, 28GST was localized in tegumental syncytium, cytons, parenchyma, and sperm tails examined by immunoelectron microscopy. C. sinensis 28GST was earlier found to neutralize bioreactive compounds and to be rich in eggs. Accordingly, it is suggested that 28GST plays important roles in phase II defense system and physiological roles in worm fecundity of C. sinensis.
摘要
谷胱甘肽S-转移酶（28GST）分子量为28千道尔顿，是华支睾吸虫中丰富的一种抗氧化酶。在华支睾吸虫成虫中，通过免疫电子显微镜检查发现28GST定位于皮层合胞体、细胞体、实质组织和精子尾部。较早发现华支睾吸虫28GST能中和生物活性化合物且在虫卵中含量丰富。因此，有人提出28GST在华支睾吸虫的Ⅱ相防御系统中起重要作用，并在虫体繁殖的生理过程中发挥作用。
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本文引用的文献
1
Functional specific binding of testosterone to Schistosoma haematobium 28-kilodalton glutathione S-transferase.
Infect Immun. 2002 Feb;70(2):601-5. doi: 10.1128/IAI.70.2.601-605.2002.
2
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Exp Parasitol. 2001 Apr;97(4):186-95. doi: 10.1006/expr.2001.4606.
3
Molecular cloning and characterization of a mu-class glutathione S-transferase from Clonorchis sinensis.
Mol Biochem Parasitol. 2001 Jun;115(1):69-75. doi: 10.1016/s0166-6851(01)00270-5.
4
Secretion of glutathione S-transferase isoforms in the seminiferous tubular fluid, tissue distribution and sex steroid binding by rat GSTM1.
Biochem J. 1999 May 15;340 ( Pt 1)(Pt 1):309-20.
5
Immunolocalisation of the glutathione S-transferases, GST-26 and GST-28, within adult Schistosoma japonicum.
Int J Parasitol. 1998 Sep;28(9):1437-43. doi: 10.1016/s0020-7519(98)00115-5.
6
Schistosoma mansoni: the developmental regulation and immunolocalization of antioxidant enzymes.
Exp Parasitol. 1997 May;86(1):69-78. doi: 10.1006/expr.1997.4150.
7
Determination of a binding site for a non-substrate ligand in mammalian cytosolic glutathione S-transferases by means of fluorescence-resonance energy transfer.
Eur J Biochem. 1996 Oct 15;241(2):484-8. doi: 10.1111/j.1432-1033.1996.00484.x.
8
Ultrastructural localization of Sm28 GST protective antigen in Schistosoma mansoni adult worms.
Parasitology. 1996 Oct;113 ( Pt 4):377-91. doi: 10.1017/s003118200006652x.
9
The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance.
Crit Rev Biochem Mol Biol. 1995;30(6):445-600. doi: 10.3109/10409239509083491.
10
Purification and characterization of prostaglandin-H E-isomerase, a sigma-class glutathione S-transferase, from Ascaridia galli.
Biochem J. 1996 Jan 1;313 ( Pt 1)(Pt 1):223-7. doi: 10.1042/bj3130223.
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