果蝇的HOAP蛋白是端粒封端所必需的。

The Drosophila HOAP protein is required for telomere capping.

作者信息

Cenci Giovanni, Siriaco Giorgia, Raffa Grazia D, Kellum Rebecca, Gatti Maurizio

机构信息

Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, 00185 Rome, Italy.

出版信息

Nat Cell Biol. 2003 Jan;5(1):82-4. doi: 10.1038/ncb902.

DOI:10.1038/ncb902

PMID:12510197

Abstract

HOAP (HP1/ORC-associated protein) has recently been isolated from Drosophila melanogaster embryos as part of a cytoplasmic complex that contains heterochromatin protein 1 (HP1) and the origin recognition complex subunit 2 (ORC2). Here, we show that caravaggio, a mutation in the HOAP-encoding gene, causes extensive telomere-telomere fusions in larval brain cells, indicating that HOAP is required for telomere capping. Our analyses indicate that HOAP is specifically enriched at mitotic chromosome telomeres, and strongly suggest that HP1 and HOAP form a telomere-capping complex that does not contain ORC2.

摘要

HOAP（HP1/ORC相关蛋白）最近从黑腹果蝇胚胎中分离出来，它是一种细胞质复合物的一部分，该复合物包含异染色质蛋白1（HP1）和起源识别复合物亚基2（ORC2）。在这里，我们表明，卡拉瓦乔（caravaggio），即HOAP编码基因中的一个突变，会导致幼虫脑细胞中广泛的端粒-端粒融合，这表明HOAP是端粒封端所必需的。我们的分析表明，HOAP在有丝分裂染色体端粒处特异性富集，并强烈表明HP1和HOAP形成了一个不包含ORC2的端粒封端复合物。