舒林酸诱导HT-29结肠腺癌细胞凋亡

[Sulindac induced apoptosis of HT-29 colon adenocarcinoma cell].

作者信息

Yang Z, Ouyang Q, Wei Y

机构信息

Department of Gastroenterology, First Affiliated Hospital, WCUMS, Chengdu 610041.

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 2000 Jun;31(2):177-9.

PMID:12515128

Abstract

This study was intended to examine whether sulindac induces apoptosis in the HT-29 cells. Flow cytometry, transmission electron microscopy and DNA electrophoresis were used. Flow cytometry, transmission electron microscopy and DNA electrophoresis all demonstrated that sulindac could induce apoptosis of the HT-29 cell line in a time- and dose-dependent manner. After treatment with 0.3 mmol/L, 0.6 mmol/L and 1.2 mmol/L sulindac for 48 hours, apoptotic cells in the treatment groups reached 5.8%, 7.6% and 11.7% versus 2.9% in the control group; after treatment for 72 hours, apoptotic cells in the treatment groups increased to 12.5%, 15.4% and 24.4% versus 5.1% in the control group, respectively, P < 0.05. This study has demonstrated that colon adenocarcinoma HT-29 cell line can be induced by sulindac.

摘要

本研究旨在探讨舒林酸是否能诱导HT - 29细胞凋亡。采用了流式细胞术、透射电子显微镜和DNA电泳技术。流式细胞术、透射电子显微镜和DNA电泳均表明，舒林酸能以时间和剂量依赖性方式诱导HT - 29细胞系凋亡。用0.3 mmol/L、0.6 mmol/L和1.2 mmol/L舒林酸处理48小时后，治疗组凋亡细胞分别达到5.8%、7.6%和11.7%，而对照组为2.9%；处理72小时后，治疗组凋亡细胞分别增至12.5%、15.4%和24.4%，而对照组为5.1%，P < 0.05。本研究表明，舒林酸可诱导结肠腺癌HT - 29细胞系凋亡。