Wu Wenxin, Zhang Xianghong, Yan Xia, Wang Junling, Zhang Jieying, Li Yuehong
Department of Pathology, Second Hospital, Hebei Medical University, Shijiazhuang 050000, China.
Zhonghua Zhong Liu Za Zhi. 2002 May;24(3):264-7.
Research was done on the possible roles of beta-catenin, p53 and proliferating cell nuclear antigen (PCNA) in the carcinogenesis of colorectal adenoma (CRA).
beta-catenin and p53 and PCNA expressions were studied with immunohistochemical stain in 77 specimens of CRA together with mild epithelial dysplasia (CRA-MD), CRA with moderate/severe epithelial dysplasia (CRA-D/SD) and CRA with cancerous changes (CRA-C).
The percentage of abnormal expression of beta-catenin increased during the transition from CRA-MD to CRA-D/SD to CRA-C (P < 0.01). The nuclear expressions of beta-catenin in CRA-D/SD and CRA-C were all significantly higher than that in CRA-MD. Expression of p53 and PCNA were increased from CRA-MD to CRA-D/SD to CRA-C, with the positive rates in these three groups of 10.3%, 43.8%, 75.0% for p53 and 17.2%, 62.5%, 87.5% for PCNA, respectively. 69.7% of cases with positive nuclear beta-catenin expression showed strong PCNA positivity which was much higher than the 36.4% of cases without nuclear beta-catenin expression (P < 0.05). The percentage of strong PCNA expression in the p53 positive cases was significant higher than that in cases with negative p53 expression (72.4% vs 37.5%, P < 0.05). Nuclear beta-catenin and p53 co-expression rates in CRA-C reached 50%.
beta-catenin, p53 and PCNA may play important roles in the carcinogenesis of colorectal adenoma.
研究β-连环蛋白、p53和增殖细胞核抗原（PCNA）在大肠腺瘤（CRA）发生过程中的可能作用。
采用免疫组织化学染色法，对77例伴有轻度上皮异型增生的CRA（CRA-MD）、伴有中/重度上皮异型增生的CRA（CRA-D/SD）和伴有癌变的CRA（CRA-C）标本进行β-连环蛋白、p53和PCNA表达研究。
从CRA-MD到CRA-D/SD再到CRA-C的转变过程中，β-连环蛋白异常表达的百分比增加（P<0.01）。CRA-D/SD和CRA-C中β-连环蛋白的核表达均显著高于CRA-MD。p53和PCNA的表达从CRA-MD到CRA-D/SD再到CRA-C逐渐增加，这三组中p53的阳性率分别为10.3%、43.8%、75.0%，PCNA的阳性率分别为17.2%、62.5%、87.5%。β-连环蛋白核表达阳性的病例中69.7%显示PCNA强阳性，这一比例远高于β-连环蛋白核表达阴性病例中的36.4%（P<0.05）。p53阳性病例中PCNA强表达的百分比显著高于p53阴性病例（72.4%对37.5%，P<0.05）。CRA-C中β-连环蛋白和p53的共表达率达到50%。
β-连环蛋白、p53和PCNA可能在大肠腺瘤的发生中起重要作用。
