[β-连环蛋白、p53及增殖细胞核抗原在大肠腺瘤癌变中的表达]

[Expressions of beta-catenin, p53 and proliferating cell nuclear antigen in the carcinogenesis of colorectal adenoma].

作者信息

Wu Wenxin, Zhang Xianghong, Yan Xia, Wang Junling, Zhang Jieying, Li Yuehong

机构信息

Department of Pathology, Second Hospital, Hebei Medical University, Shijiazhuang 050000, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2002 May;24(3):264-7.

PMID:12515622

Abstract

OBJECTIVE

Research was done on the possible roles of beta-catenin, p53 and proliferating cell nuclear antigen (PCNA) in the carcinogenesis of colorectal adenoma (CRA).

METHODS

beta-catenin and p53 and PCNA expressions were studied with immunohistochemical stain in 77 specimens of CRA together with mild epithelial dysplasia (CRA-MD), CRA with moderate/severe epithelial dysplasia (CRA-D/SD) and CRA with cancerous changes (CRA-C).

RESULTS

The percentage of abnormal expression of beta-catenin increased during the transition from CRA-MD to CRA-D/SD to CRA-C (P < 0.01). The nuclear expressions of beta-catenin in CRA-D/SD and CRA-C were all significantly higher than that in CRA-MD. Expression of p53 and PCNA were increased from CRA-MD to CRA-D/SD to CRA-C, with the positive rates in these three groups of 10.3%, 43.8%, 75.0% for p53 and 17.2%, 62.5%, 87.5% for PCNA, respectively. 69.7% of cases with positive nuclear beta-catenin expression showed strong PCNA positivity which was much higher than the 36.4% of cases without nuclear beta-catenin expression (P < 0.05). The percentage of strong PCNA expression in the p53 positive cases was significant higher than that in cases with negative p53 expression (72.4% vs 37.5%, P < 0.05). Nuclear beta-catenin and p53 co-expression rates in CRA-C reached 50%.

CONCLUSION

beta-catenin, p53 and PCNA may play important roles in the carcinogenesis of colorectal adenoma.

摘要

目的

研究β-连环蛋白、p53和增殖细胞核抗原（PCNA）在大肠腺瘤（CRA）发生过程中的可能作用。

方法

采用免疫组织化学染色法，对77例伴有轻度上皮异型增生的CRA（CRA-MD）、伴有中/重度上皮异型增生的CRA（CRA-D/SD）和伴有癌变的CRA（CRA-C）标本进行β-连环蛋白、p53和PCNA表达研究。

结果

从CRA-MD到CRA-D/SD再到CRA-C的转变过程中，β-连环蛋白异常表达的百分比增加（P<0.01）。CRA-D/SD和CRA-C中β-连环蛋白的核表达均显著高于CRA-MD。p53和PCNA的表达从CRA-MD到CRA-D/SD再到CRA-C逐渐增加，这三组中p53的阳性率分别为10.3%、43.8%、75.0%，PCNA的阳性率分别为17.2%、62.5%、87.5%。β-连环蛋白核表达阳性的病例中69.7%显示PCNA强阳性，这一比例远高于β-连环蛋白核表达阴性病例中的36.4%（P<0.05）。p53阳性病例中PCNA强表达的百分比显著高于p53阴性病例（72.4%对37.5%，P<0.05）。CRA-C中β-连环蛋白和p53的共表达率达到50%。