Zheng Jiang, Zhou Hong, Lu Yongling, Xiao Guangxia
Medical Research Center, Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038, P.R. China.
Zhonghua Shao Shang Za Zhi. 2002 Apr;18(2):95-8.
To observe the role of four modeling peptides (10342, 10343, 10344, 10345) derived from bactericidal/permeability increasing protein (BPI) in neutralizing endotoxin (LPS) in vitro and in vivo.
Quantitative limulus amoebocyte lysate assay was employed to evaluate the capacity of BPI peptides in neutralizing endotoxin in vitro. The protective capacity of the peptides was observed in mice challenged with endotoxin by intravenous administration via the tail vein. The influence of the peptides on serum TNFalpha and IL-6 levels in rats with endotoxemia were also observed.
All of the four peptides possessed endotoxin-neutralizing capacity which was strengthened along with the increase in their concentration. Among the peptides, 10342 is the strongest one. All of the peptides had strong power to protect mice from endotoxin with 90% protective rate. In the rats with endotoxemia, the four peptides could reduce the levels of serum TNFalpha and IL-6 significantly at different time-points.
Four BPI modeling peptides possessed not only endotoxin-neutralizing capacity in vitro, but also potential protective capacity in animals challenged with endotoxin.
观察源自杀菌/通透性增加蛋白（BPI）的四种模拟肽（10342、10343、10344、10345）在体内外中和内毒素（LPS）中的作用。
采用定量鲎试剂法评估BPI肽体外中和内毒素的能力。通过尾静脉静脉注射内毒素对小鼠进行攻击，观察肽的保护能力。还观察了肽对内毒素血症大鼠血清TNFα和IL-6水平的影响。
四种肽均具有内毒素中和能力，且随着浓度增加而增强。其中10342最强。所有肽对小鼠均有很强的保护作用，保护率达90%。在内毒素血症大鼠中，四种肽在不同时间点均可显著降低血清TNFα和IL-6水平。
四种BPI模拟肽不仅在体外具有内毒素中和能力，而且对受内毒素攻击的动物具有潜在的保护能力。
