Applegarth D A, Bozoian G, Lowry R B
Clin Biochem. 1975 Jun;8(3):151-60. doi: 10.1016/s0009-9120(75)91608-2.
Methods are presented for the small scale column chromatography of urinary mucopolysaccharides. After isolation by cety pyridinium chloride mucopolysaccharides are applied to a small column of ion exchange resin and eluted by 2 M-sodium chloride. Colorimetric assays on this elutate allow clinically significant disease states to be detected.
本文介绍了用于尿黏多糖小规模柱色谱分析的方法。通过氯化十六烷基吡啶分离后,将黏多糖应用于一小柱离子交换树脂,并用2M氯化钠洗脱。对该洗脱液进行比色测定可检测出具有临床意义的疾病状态。