Nagahata Hajime, Oota Hiromichi, Nitanai Atsushi, Oikawa Shin, Higuchi Hidetoshi, Nakade Tetsuya, Kurosawa Takashi, Morita Mitsuo, Ogawa Hiroyuki
Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
J Vet Med Sci. 2002 Dec;64(12):1107-12. doi: 10.1292/jvms.64.1107.
A female stillborn Holstein calf with shortened cervical and thoracic regions, protrusion of the tongue, and bilateral symmetric flexural contraction of the anterior limbs was delivered on gestation day 281. Multiple hemivertebrae, fused and misshaped vertebrae, synostosis and scoliosis of cervical, thoracic and lumbar vertebral column were found in the affected calf by radiographic and computed tomographic (CT) analysis. Ten pairs of ribs were present and the sternum consisted of 9 sternebrae. Multiple morphologic abnormalities including fusion, malformation, and displacement, were found in the ribs and sternum. Cardiac anomalies, including atrial septal defect and hypertrophy of right ventricle, were observed. DNA-polymerase chain reaction (PCR) analysis demonstrated that amplified product from the liver DNA of the affected calf had identical pattern to that associated with complex vertebral malformation (CVM) of Holstein calves and that her dam was a heterozygous carrier of CVM. The affected calf was diagnosed as having CVM based on the DNA-PCR results and the characteristic findings, and was recorded as a first documentation of CVM confirmed in a Holstein calf in Japan.
一头雌性荷斯坦死产犊牛于妊娠281天时产出，其颈部和胸部区域缩短，舌头突出，前肢双侧对称性弯曲挛缩。通过X线摄影和计算机断层扫描（CT）分析，在患犊中发现颈椎、胸椎和腰椎存在多个半椎体、融合及形态异常的椎体、椎体间骨性连接和脊柱侧弯。有十对肋骨，胸骨由九块胸骨节组成。在肋骨和胸骨中发现了包括融合、畸形和移位在内的多种形态异常。观察到心脏异常，包括房间隔缺损和右心室肥厚。DNA聚合酶链反应（PCR）分析表明，患犊肝脏DNA的扩增产物与荷斯坦犊牛复杂椎体畸形（CVM）相关的模式相同，其母为CVM的杂合携带者。根据DNA-PCR结果和特征性表现诊断该患犊患有CVM，这被记录为日本荷斯坦犊牛中确诊的CVM的首例记录。
