Felix J M, Sutter-Dub M T, Legrele C, Billaudel B
Horm Metab Res. 1975 Jul;7(4):309-14. doi: 10.1055/s-0028-1093720.
The double antibody procedure detects 3 peaks in the elution fractions of adult or fetal rat sera, after passage on Sephadex G50 or G100 columns. Peak A (apparent MW 6000) contains insulin monomer; peak B (apparent MW 10-12000) is tentatively attributed to proinsulin (or proinsulin like substances); peak C (apparent MW 50-100000) is similar to the so called "big big" insulin. During intravenously induced hyperglycemia, the 3 peaks show parallel increases, but, after the disappearance of peaks A and B in streptozotocin treated rats, peak C remains unaltered. Pancreatic extracts and secreta present a very minor and inconstant peak C, the bulk of their immunoreactive material belonging to peaks A and B. A companion paper further discusses the nature of peaks B and C materials.
双抗体法检测成年或胎鼠血清经Sephadex G50或G100柱洗脱后的组分中有3个峰。峰A（表观分子量6000）含有胰岛素单体；峰B（表观分子量10 - 12000）暂归因于胰岛素原（或类胰岛素原物质）；峰C（表观分子量50 - 100000）类似于所谓的“大大”胰岛素。静脉注射诱导高血糖期间，这3个峰呈平行增加，但在链脲佐菌素处理的大鼠中峰A和峰B消失后，峰C保持不变。胰腺提取物和分泌物呈现出非常小且不稳定的峰C，其大部分免疫反应性物质属于峰A和峰B。一篇相关论文进一步讨论了峰B和峰C物质的性质。
