Cheng Z J, Zhao L, Tian H, Cong T, Li Z, Bao S F
Trace Element Research Lab, General Hospital of PLA, Beijing 100853, China.
Zhongguo Zhong Yao Za Zhi. 2001 Mar;26(3):194-7.
To investigate the changes of copper, zinc and selenium levels in rat tissues after long-term oral administration of Realgar.
Rats were given Realgar with dosage of 50, 150, 450 mg.kg-1.d-1 for 5 weeks, and the concentrations of copper, zinc and selenium in different rat tissues as well as the contents of metallothionein in rat liver and kidney were determined with atomic absorption and hydride generation-atomic fluorescent technique. The total amount of copper excreted from feces and urine of each rat 5 days before the rat was killed was also measured.
No significant changes of levels of copper, zinc and selenium in rat tissues were found after administration of low and middle dosages of Realgar. But higher dosage (450 mg.kg-1.d-1) of Realgar administration could induce a small but significant decrease of zinc concentration in hearts and a increase of copper contents in spleen and tibia, as well as twice more copper concentration of kidney.
Copper deposit in kidney was the most significant change found among the trace elements levels in rat tissues, and this might be one of the mechanisms for kidney toxicity of Realgar.
研究长期口服雄黄后大鼠组织中铜、锌、硒含量的变化。
将大鼠按50、150、450mg·kg-1·d-1的剂量给予雄黄，连续给药5周，采用原子吸收法和氢化物发生-原子荧光法测定大鼠不同组织中铜、锌、硒的含量以及肝脏和肾脏中金属硫蛋白的含量。同时测定处死前5天每只大鼠粪便和尿液中铜的排泄总量。
低、中剂量雄黄给药后，大鼠组织中铜、锌、硒含量无明显变化。但高剂量（450mg·kg-1·d-1）雄黄给药可导致心脏中锌浓度轻度但显著降低，脾脏和胫骨中铜含量增加，肾脏中铜浓度升高两倍。
肾脏中铜沉积是大鼠组织微量元素水平变化中最显著的改变，这可能是雄黄肾毒性的机制之一。
