Barrett L A, McDowell E M, Frank A L, Harris C C, Trump B F
Cancer Res. 1976 Mar;36(3):1003-10.
Human bronchial epithelium has been maintained in organ culture in serum-supplemented medium for 4 months. After 4 to 6 weeks in culture, various changes in morphology were apparent. There was an increase in autophagic vacuoles in mucous, ciliated, and basal cells, a reduction in the height of the columnar cells, a decrease in the number of goblet mucous cells, and an increase in cells with small mucous granules. After 3 months in culture, the basal lamina was frequently covered by 2 or 3 layers of epithelial cells consisting of nonkeratinizing squamous cells with short microvilli and small mucous granules. Less frequently, keratinizing squamous cells were seen. Differentiated epithelium incorporated precursors into macromolecules in serum-free medium, supplemented with vitamin A, at 1 week of culture. These explants exhibited changed epithelium by 2 weeks, similar to that described for epithelium in serum-supplemented medium after 4 to 6 weeks.
人支气管上皮细胞在添加血清的培养基中进行器官培养已达4个月。培养4至6周后，形态上出现了各种变化。黏液细胞、纤毛细胞和基底细胞中的自噬泡增多，柱状细胞高度降低，杯状黏液细胞数量减少，具有小黏液颗粒的细胞增多。培养3个月后，基膜常被2或3层上皮细胞覆盖，这些上皮细胞由具有短微绒毛和小黏液颗粒的非角化鳞状细胞组成。较少见角化鳞状细胞。在培养1周时，分化的上皮细胞在补充了维生素A的无血清培养基中将前体整合到大分子中。这些外植体在2周时上皮细胞发生了变化，类似于在添加血清的培养基中培养4至6周后的上皮细胞变化。
