Hall S H, Smuckler E A
Cancer Res. 1976 Mar;36(3):1094-1100.
DNA-dependent RNA polymerase A (or 1) was purified from murine myeloma MOPC 21 by diethylaminoethyl Sephadex chromatography. Further separation from DNA polymerases, protein kinase and DNA endonuclease was accomplished by polyriboadenylate-Sepharose affinity chromatography followed by gradient centrifugation. Yields following chromatography were 100%, but following gradient centrifugation only 25 to 30% of the activity remained. Addition of low-molecular-weight components increased yields to 50 to 60%. Several species of myeloma polymerase A could be detected, and subunits of 190,000 and 125,000 daltons were identified. No evidence of phosphorylation of the polymerase was found.
通过二乙氨基乙基葡聚糖凝胶色谱法从鼠骨髓瘤MOPC 21中纯化出依赖DNA的RNA聚合酶A（或1）。通过聚核糖腺苷酸-琼脂糖亲和色谱法，随后进行梯度离心，进一步从DNA聚合酶、蛋白激酶和DNA内切核酸酶中分离出来。色谱分离后的产率为100%，但梯度离心后仅保留25%至30%的活性。添加低分子量成分可将产率提高到50%至60%。可检测到几种骨髓瘤聚合酶A，鉴定出了190,000和125,000道尔顿的亚基。未发现聚合酶磷酸化的证据。
