• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Genetic and biochemical studies of phosphatase activity of PhoR.PhoR磷酸酶活性的遗传与生化研究。
J Bacteriol. 2003 Feb;185(3):1112-5. doi: 10.1128/JB.185.3.1112-1115.2003.
2
Decay of activated Bacillus subtilis pho response regulator, PhoP approximately P, involves the PhoR approximately P intermediate.活化的枯草芽孢杆菌 Pho 应答调节因子 PhoP~P 的衰变涉及 PhoR~P 中间体。
Biochemistry. 1999 Aug 3;38(31):10119-25. doi: 10.1021/bi990658t.
3
Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2.PhoM蛋白与大肠杆菌磷酸调节子的相互作用：PhoM是一种组氨酸蛋白激酶，催化PhoB和PhoM开放阅读框2的磷酸化。
J Bacteriol. 1990 Nov;172(11):6300-7. doi: 10.1128/jb.172.11.6300-6307.1990.
4
The cytoplasmic kinase domain of PhoR is sufficient for the low phosphate-inducible expression of pho regulon genes in Bacillus subtilis.PhoR的胞质激酶结构域足以在枯草芽孢杆菌中实现低磷诱导的pho调控子基因表达。
Mol Microbiol. 1999 Jan;31(1):211-22. doi: 10.1046/j.1365-2958.1999.01163.x.
5
Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins.大肠杆菌磷酸调节子中的信号转导涉及PhoR和PhoB蛋白之间的磷酸转移。
J Mol Biol. 1989 Dec 5;210(3):551-9. doi: 10.1016/0022-2836(89)90131-9.
6
Probing kinase and phosphatase activities of two-component systems in vivo with concentration-dependent phosphorylation profiling.采用浓度依赖型磷酸化谱法在体内探测双组分系统的激酶和磷酸酶活性。
Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):672-7. doi: 10.1073/pnas.1214587110. Epub 2012 Dec 24.
7
Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon.新型方法用于构建严格调控的阿拉伯糖和鼠李糖启动子融合体在大肠杆菌磷酸盐调节子研究中的应用
J Bacteriol. 1998 Mar;180(5):1277-86. doi: 10.1128/JB.180.5.1277-1286.1998.
8
Bacillus subtilis PhoP binds to the phoB tandem promoter exclusively within the phosphate starvation-inducible promoter.枯草芽孢杆菌PhoP仅在磷酸盐饥饿诱导型启动子内与phoB串联启动子结合。
J Bacteriol. 1997 Oct;179(20):6302-10. doi: 10.1128/jb.179.20.6302-6310.1997.
9
Crystal structure of the catalytic ATP-binding domain of the PhoR sensor histidine kinase.PhoR 传感器组氨酸激酶催化 ATP 结合域的晶体结构。
Proteins. 2023 Jul;91(7):999-1004. doi: 10.1002/prot.26473. Epub 2023 Feb 17.
10
A Dual Regulatory Role of the PhoU Protein in Salmonella Typhimurium.PhoU 蛋白在鼠伤寒沙门氏菌中的双重调控作用。
mBio. 2022 Jun 28;13(3):e0081122. doi: 10.1128/mbio.00811-22. Epub 2022 May 31.

引用本文的文献

1
Roles of Pho regulon in bacterial pathogenicity.Pho 调控子在细菌致病性中的作用。
Virulence. 2025 Dec;16(1):2545559. doi: 10.1080/21505594.2025.2545559. Epub 2025 Aug 13.
2
Regulatory mechanism of the SenX3-RegX3 two-component system in Mycobacterium smegmatis: Roles of PhoU in sensing inorganic phosphate levels.耻垢分枝杆菌中SenX3-RegX3双组分系统的调控机制：PhoU在感知无机磷酸盐水平中的作用。
J Biol Chem. 2025 Jun 28;301(8):110435. doi: 10.1016/j.jbc.2025.110435.
3
Comparison of Phenotype Nutritional Profiles and Phosphate Metabolism Genes in Four Serovars of from Water Sources.水源中四种血清型的表型营养特征与磷酸盐代谢基因的比较。
Microorganisms. 2023 Aug 18;11(8):2109. doi: 10.3390/microorganisms11082109.
4
A Survey of Two-Component Systems in Coxiella burnetii Reveals Redundant Regulatory Schemes and a Requirement for an Atypical PhoBR System in Mammalian Cell Infection.考克斯氏体中二组分系统的研究揭示了冗余的调控方案和在哺乳动物细胞感染中需要一个非典型的 PhoBR 系统。
J Bacteriol. 2023 Mar 21;205(3):e0041622. doi: 10.1128/jb.00416-22. Epub 2023 Feb 27.
5
Prospects for Using Phosphate-Solubilizing Microorganisms as Natural Fertilizers in Agriculture.利用解磷微生物作为农业天然肥料的前景
Plants (Basel). 2022 Aug 15;11(16):2119. doi: 10.3390/plants11162119.
6
Whole-Genome Sequencing and Comparative Genomic Analysis of Antimicrobial Producing from the Rumen.瘤胃中产抗菌物质微生物的全基因组测序及比较基因组分析
Microorganisms. 2022 Mar 3;10(3):551. doi: 10.3390/microorganisms10030551.
7
Coordination of Phosphate and Magnesium Metabolism in Bacteria.细菌中磷酸盐和镁代谢的协调。
Adv Exp Med Biol. 2022;1362:135-150. doi: 10.1007/978-3-030-91623-7_12.
8
Strategies of organic phosphorus recycling by soil bacteria: acquisition, metabolism, and regulation.土壤细菌有机磷循环策略：获取、代谢和调控。
Environ Microbiol Rep. 2022 Feb;14(1):3-24. doi: 10.1111/1758-2229.13040. Epub 2022 Jan 10.
9
Stochastic Simulations as a Tool for Assessing Signal Fidelity in Gene Expression in Synthetic Promoter Design.随机模拟作为评估合成启动子设计中基因表达信号保真度的工具
Biology (Basel). 2021 Jul 29;10(8):724. doi: 10.3390/biology10080724.
10
Effect of Varying Nitrate Concentrations on Denitrifying Phosphorus Uptake by DPAOs With a Molecular Insight Into Pho Regulon Gene Expression.不同硝酸盐浓度对反硝化聚磷菌吸收磷的影响及对Pho调控基因表达的分子洞察
Front Microbiol. 2019 Nov 8;10:2586. doi: 10.3389/fmicb.2019.02586. eCollection 2019.

本文引用的文献

1
Two-component signal transduction.双组分信号转导
Annu Rev Biochem. 2000;69:183-215. doi: 10.1146/annurev.biochem.69.1.183.
2
Phosphatase activity of histidine kinase EnvZ without kinase catalytic domain.无激酶催化结构域的组氨酸激酶EnvZ的磷酸酶活性
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7808-13. doi: 10.1073/pnas.97.14.7808.
3
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.利用PCR产物一步灭活大肠杆菌K-12中的染色体基因。
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. doi: 10.1073/pnas.120163297.
4
Histidine kinases: diversity of domain organization.组氨酸激酶：结构域组织的多样性
Mol Microbiol. 1999 Nov;34(4):633-40. doi: 10.1046/j.1365-2958.1999.01646.x.
5
Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ.大肠杆菌组氨酸激酶EnvZ同二聚体核心结构域的溶液结构
Nat Struct Biol. 1999 Aug;6(8):729-34. doi: 10.1038/11495.
6
PAS domains: internal sensors of oxygen, redox potential, and light.PAS结构域：氧气、氧化还原电位和光的内部传感器。
Microbiol Mol Biol Rev. 1999 Jun;63(2):479-506. doi: 10.1128/MMBR.63.2.479-506.1999.
7
Autophosphorylation, phosphotransfer, and DNA-binding properties of the RegB/RegA two-component regulatory system in Rhodobacter capsulatus.荚膜红细菌中RegB/RegA双组分调节系统的自磷酸化、磷酸转移及DNA结合特性
J Biol Chem. 1999 Jun 4;274(23):16343-8. doi: 10.1074/jbc.274.23.16343.
8
Analysis of the conserved acidic residues in the regulatory domain of PhoB.
FEBS Lett. 1998 Dec 18;441(2):242-6. doi: 10.1016/s0014-5793(98)01556-7.
9
NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ.大肠杆菌渗透压感受器EnvZ组氨酸激酶结构域的核磁共振结构
Nature. 1998 Nov 5;396(6706):88-92. doi: 10.1038/23968.
10
Two-domain reconstitution of a functional protein histidine kinase.功能性蛋白质组氨酸激酶的双结构域重组
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6728-32. doi: 10.1073/pnas.95.12.6728.

PhoR磷酸酶活性的遗传与生化研究。

Genetic and biochemical studies of phosphatase activity of PhoR.

作者信息

Carmany Daniel O, Hollingsworth Kristine, McCleary William R

机构信息

Microbiology and Molecular Biology Department, Brigham Young University, Provo, UT 84602-5253, USA.

出版信息

J Bacteriol. 2003 Feb;185(3):1112-5. doi: 10.1128/JB.185.3.1112-1115.2003.

DOI:10.1128/JB.185.3.1112-1115.2003
PMID:12533489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC142828/
Abstract

In Escherichia coli, PhoR is the histidine kinase of the phosphate regulon. It has been postulated that PhoR may function as a phospho-PhoB phosphatase. Experiments with four precise phoR deletion mutants supported this hypothesis and suggested that this activity resides within the histidine phosphorylation domain. This biochemical activity was confirmed by using a separately expressed histidine phosphorylation domain.

摘要

在大肠杆菌中，PhoR是磷酸调节子的组氨酸激酶。据推测，PhoR可能作为磷酸化PhoB磷酸酶发挥作用。对四个精确的phoR缺失突变体进行的实验支持了这一假设，并表明这种活性存在于组氨酸磷酸化结构域内。通过使用单独表达的组氨酸磷酸化结构域证实了这种生化活性。