Guzińska-Ustymowicz Katarzyna, Famulski Waldemar, Stasiak-Barmuta Anna, Zalewski Bogdan, Sulkowska Mariola
Department of Clinical Pathomorphology, Medical Academy of Białystok, Białystok, Poland.
Rocz Akad Med Bialymst. 2002;47:246-53.
The aim of our study was to elucidate whether and in a what way applying two slightly different immunohistochemical procedures effects Bcl-2 protein detection as well as its relationship to colorectal cancer clinicopathological features. A series of 74 primary colorectal cancers was investigated for expression of the Bcl-2 protein. Two immunohistochemical procedures: first with antigen retrieval using a microwave (art-), second with antigen retrieval by use pressure pot (art+) pre-treatment, were applied in each cancer case. Formalin-fixed, paraffin embedded tissues were treated with ant-Bcl-2 antibody (Dako No M 0887). Bcl-2 (art+) immunostaining was positive in 48 (64.9%) cases of colorectal cancers whereas Bcl-2 (art-) immunohistochemical reaction was positive only in 15 (20%) colorectal tumours. A significant, positive correlation was observed between Bcl-2 (art+) expression and localization of the tumour in the rectum (p = 0.007). At the same time a statistically significant positive correlation between tumour Bcl-2 (art-) expression and colon tumour pT stage (p = 0.007), rectum tumour differentiation grade (p = 0.04) and Bcl-2 (art-) protein immunostaining in lymph node metastases (p < 0.000001) was observed. This data suggest that the type of procedure for antigen retrieval to detection of Bcl-2 protein by immunohistochemical reaction, changes the study results and should be considered when comparing own results with the literature data.
我们研究的目的是阐明采用两种略有不同的免疫组织化学方法是否以及以何种方式影响Bcl-2蛋白检测及其与结直肠癌临床病理特征的关系。对74例原发性结直肠癌进行了Bcl-2蛋白表达研究。对每个癌症病例应用两种免疫组织化学方法：第一种是使用微波进行抗原修复（art-），第二种是使用高压锅进行抗原修复（art+）预处理。用抗Bcl-2抗体（Dako No M 0887）处理福尔马林固定、石蜡包埋的组织。Bcl-2（art+）免疫染色在48例（64.9%）结直肠癌病例中呈阳性，而Bcl-2（art-）免疫组化反应仅在15例（20%）结直肠肿瘤中呈阳性。观察到Bcl-2（art+）表达与肿瘤在直肠中的定位之间存在显著的正相关（p = 0.007）。同时，观察到肿瘤Bcl-2（art-）表达与结肠肿瘤pT分期（p = 0.007）、直肠肿瘤分化程度（p = 0.04）以及淋巴结转移中的Bcl-2（art-）蛋白免疫染色之间存在统计学上显著的正相关（p < 0.000001）。这些数据表明，免疫组化反应检测Bcl-2蛋白时抗原修复的方法类型会改变研究结果，在将自己的结果与文献数据进行比较时应予以考虑。
