代谢功能多样的恶臭假单胞菌KT2440的全基因组序列及比较分析
Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.
作者信息
Nelson K E, Weinel C, Paulsen I T, Dodson R J, Hilbert H, Martins dos Santos V A P, Fouts D E, Gill S R, Pop M, Holmes M, Brinkac L, Beanan M, DeBoy R T, Daugherty S, Kolonay J, Madupu R, Nelson W, White O, Peterson J, Khouri H, Hance I, Chris Lee P, Holtzapple E, Scanlan D, Tran K, Moazzez A, Utterback T, Rizzo M, Lee K, Kosack D, Moestl D, Wedler H, Lauber J, Stjepandic D, Hoheisel J, Straetz M, Heim S, Kiewitz C, Eisen J A, Timmis K N, Düsterhöft A, Tümmler B, Fraser C M
机构信息
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
出版信息
Environ Microbiol. 2002 Dec;4(12):799-808. doi: 10.1046/j.1462-2920.2002.00366.x.
Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.
恶臭假单胞菌是一种代谢功能多样的腐生土壤细菌，已被认证为用于克隆外源基因的生物安全宿主。这种细菌在生物技术应用方面也具有相当大的潜力。对KT2440菌株6.18 Mb基因组的序列分析揭示了多样的转运和代谢系统。尽管与致病性假单胞菌铜绿假单胞菌的基因组保守程度较高（85%的预测编码区域是共享的），但包括外毒素A和III型分泌系统在内的关键毒力因子并不存在。对该基因组的分析有助于深入了解恶臭假单胞菌的非致病特性，并指出其在农业、生物催化、生物修复和生物塑料生产方面潜在的新应用。
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