[通过假型逆转录病毒载体将高效基因导入乳腺癌细胞]
[High efficiency gene transfer into breast cancer cells by pseudotyped retroviral vector].
作者信息
Tang L L, Yu H
机构信息
Department of Oncology, Xiangya Hospital, Central South University, Changsha, 41008.
出版信息
Hunan Yi Ke Da Xue Xue Bao. 2001 Feb 28;26(1):48-50.
OBJECTIVE
To investigate the efficiency of gene transfer mediated by pseudotyped retroviral vector in breast cancer, and to provide an useful vector for breast cancer gene therapy.
METHODS
We used a MuLV vector pseudotyped with the vesicular stomatitis virus glycoprotein(VSV-G) envelope to transduce breast cancer cells MDA-MB-435, To compare the transfer efficiency with MuLV.
RESULTS
The transfer efficiency is (92 +/- 12)% by using MuLV/VSV-G vector, (24 +/- 5)% by MuLV vector. The transfer efficiency of MuLV/VSV-G is 3.8 times that of MuLV vector.
CONCLUSION
Our results suggest that pseudotyped retroviral vector MuLV/VSV-G is a kind of high efficiency gene transfer vector in breast cancer cells.
目的
研究假型逆转录病毒载体介导的基因转移在乳腺癌中的效率，为乳腺癌基因治疗提供一种有用的载体。
方法
我们使用了一种用水泡性口炎病毒糖蛋白（VSV-G）包膜假型化的莫洛尼鼠白血病病毒（MuLV）载体来转导乳腺癌细胞MDA-MB-435，以与MuLV比较转移效率。
结果
使用MuLV/VSV-G载体的转移效率为（92±12）%，使用MuLV载体的转移效率为（24±5）%。MuLV/VSV-G的转移效率是MuLV载体的3.8倍。
结论
我们的结果表明，假型逆转录病毒载体MuLV/VSV-G是一种在乳腺癌细胞中高效的基因转移载体。
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